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NGS Library Prep

19 products

Showing 1 - 19 of 19 products

Discover Yeasen’s comprehensive portfolio of next-generation sequencing (NGS) reagents, including high-performance library preparation kits, enzymes for DNA/RNA processing, and magnetic beads for clean-up and size selection. Trusted worldwide, our kits offer high quality and speed; e.g., Total RNA Library Prep Kit (Cat#12341) uses just 5 tubes and saves over 2 hours.

The 3rd Generation Sequencing
Application	Cat. No.	Description
gDNA Sequencing	13301ES	DNA Library Prep
Amplicon & PCR Products Library Prep	13306ES	End Repair & Ligation Module
	13304ES	Adapter Ligation Module
	13317–13320ES	Native barcode kits, 384 Barcode
Plasmid Library Prep	13305ES	Fragmentation & End Repair & Ligation Module
	13304ES	Adapter Ligation Module
	13317–13320ES	Native barcode kits, 384 Barcode
Full-Length RNA Library Prep	12937ES	Full-length cDNA Library Prep
	13302ES	End Repair & Ligation Module
	13304ES	Adapter Ligation Module
rRNA Depletion	12266ES	rRNA Removing for Mammalian Samples(Magnetic method)
	12264ES	rRNA Removing for Bacterial Samples(Magnetic method)
	12262ES	rRNA Removing for Plant Samples(Magnetic method)

NGS Reagents for Academy and Agriculture
Application	Name	Cat:	Specification
DNA Lib Prep	Hieff NGS™ DNA Library Prep Kit 2.0	12927	Mechanical
	Hieff NGS™ OnePot Pro DNA Library Prep Kit V4	12972	Enzymatic
	Hieff NGS™ OnePot EvoMax DNA Library Prep Kit(Premixed Version)	12112	Ultra-simple premix: 3 tubes
Total RNA Lib Prep Premix	Hieff NGS™ EvoMax RNA Library Prep Kit	12341	Strand specific, 5 tubes
Total RNA Lib Prep Premix	Hieff NGS™ EvoMax RNA Library Prep Kit (dUTP)	12340	Non-strand, 5 tubes
Regular RNA Lib Prep	Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit	12308	Strand specific & Non Strand specific
mRNA Isolation	Hieff NGS™ mRNA Isolation Master Kit V2	12629
rRNA Depletion	Hieff NGS™ MaxUp Human rRNA Depletion Kit	12257	Human/Mouse/Rat
	Hieff NGS™ MaxUp rRNA Depletion Kit	12254	Plant
	Globin mRNA Depletion Probe (Human)	12806	Globin
Epigenetics	Anti-rabbit Nanobody-Tn5	12594	CUT & Tag
	Nanobody-Tn5	12595	CUT & Tag
	Hieff NGS™ DNA Spike in Mix	12596	CUT & Tag
	Concanavalin A-Coated Magnetic Beads	19810	CUT & Tag

Tumor detection
Category	Name	Cat.NO.	Application
Bisulfite Conversion	SuperMethyl™ Max Bisulfite Conversion Kit	17328ES	Cancer Early Screening
	SuperMethyl™ Fast Bisulfite Conversion Kit, Spin Column Purification	17329ES
	SuperMethyl™ Fast Bisulfite Conversion Kit, Magnetic Bead Purification	17330ES
Methylation Library Prep	Hieff™ MethyShear	12218ES
Methylation Library Prep	Hieff NGS™ dsDNA Methyl Library Prep Kit for Illumina	12214ES
cfDNA Extraction	Hieff™ Magnetic Circulating Cell-Free DNA Kit	18386ES	Liquid Biopsy
Library Prep	Hieff NGS™ DNA Library Prep Kit 2.0	12927ES	Liquid Biopsy
Blood gDNA Extraction	Hieff™ Magnetic Blood DNA Kit	18504ES	Companion Diagnostics(WES, Targeted sequencing)
Library Prep	Hieff NGS™ OnePot Evo DNA Library Prep Kit (Enzymatic, For FFPE)	12193ES
Hybrid Capture	Hieff NGS™ Human All Exon Probes	12244ES
	Hieff NGS™ Cap Beads	12248ES
	Hieff NGS™ Hyb & Wash Kit with Universal Blocking Oligo for Illumina	12245ES
	Hieff NGS™ Hyb & Wash Kit with Universal Blocking Oligo for MGI	12243ES
Multiplex PCR	2×Hieff NGS™ HG Multiplex PCR Master Mix	13283ES
Magnetic Beads	Hieff NGS™ DNA Selection Beads	12601ES

Reproductive Health Solution
Category	Name	Cat.NO.	Application
cfDNA Extraction	Hieff™ Magnetic Cell-Free DNA Kit	18386ES	-
Blood gDNA Extraction	Hieff™ Magnetic Blood DNA Kit	18504ES	-
Single-cell Whole-genome Amplification	Hieff NGS™ Single Cell/Low Input WGA Kit	12510ES	PGT-A
Library Prep	Hieff NGS™ OnePot Evo DNA Library Prep Kit (Enzymatic)	12193ES	PGT-A
Library Prep Library	Hieff NGS™ DNA Library Prep Kit 2.0	12927ES	NIPT
Library Prep Library	Hieff NGS™ OnePot Evo DNA Library Prep Kit (Enzymatic)	12193ES	WGS/WES/CNV-seq, etc.
Multiplex PCR	2×Hieff NGS™ HG Multiplex PCR Master Mix	13283ES	-
Magnetic Beads	Hieff NGS™ DNA Selection Beads	12601ES	-
Supporting Products	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set1/Set2/Set3/Set4（Plate）	12327-12330ES	Illumina UDI Adpter
	HieffNGS™ Unique Dual Barcode Primer Kit for MGI, Set1/Set2/Set3/Set4（Plate）	13350-13353ES	MGI UDB Adpter
	1×dsDNA HS Assay Kit / dsDNA HS Assay Kit	12642ES	dsDNA Quantification
	ssDNA Assay Kit	12645ES	ssDNA Quantification
	Hieff NGS™ Fast-Pace DNA Cyclization Kit	13339ES	MGI Cyclization

Library Selecion and DNA Quantification
Category	Product Name	Cat.No.	Specification
Magnetic Beads	Hieff NGS™ DNA Selection Beads	12601ES	DNA cleanup, size selection
	Hieff NGS™ Smarter DNA Clean Beads (>50 bp, compatible with ATAC and CUT&Tag)	12600ES
	Hieff NGS™ DNA Selection Beads V2 (Max Yields)	12418ES
	Hieff NGS™ mRNA Isolation Master Kit V2	12629ES	RNA cleanup
	Hieff NGS™ RNA Cleaner	12602ES	RNA cleanup
Library Quantitation	dsDNA HS Assay Kit	12640ES	0.2-100 ng, 10 pg/μL to 100 ng/μL
	1× dsDNA HS Assay Kit	12642ES	0.2-100 ng, 10 pg/μL to 100 ng/μL
	dsDNA BR Assay Kit	12643ES	2-1000 ng, 0.1 ng/µL – 1000 ng/µL
	ssDNA Assay Kit	12645ES	1-200 ng, 50 pg/µL – 200 ng/µL
	1× ssDNA Assay Kit	12646ES	1-200 ng, 50 pg/µL – 200 ng/µL
	0.5 mL thin-walled polypropylene tubes	83509ES	Consumables
	8-Tube Assay Strips (0.2mL)	83615ES	Consumables
	Nucleic Acid Quantifier Fluorometer (Single Channel)	80571ES	-
	Nucleic Acid Quantification Fluorometer Qubit (96-channel)	80575ES	-

Microbial Genomics Solution
Category	Product Name	Cat.No	Time	Amplication
	Hieff™ Magnetic Pathogen DNA/RNA Kit	18306ES	-	DNA&RNA Extraction
	Hieff™ Mag Blood Pathogen DNA/RNA Kit	18307ES	-	DNA&RNA Extraction
mNGS	Hieff NGS™ OnePot Flash DNA Library Prep Kit	12316ES	1.75 h	DNA Library for SE75/SE50
	Hieff NGS™ OnePot II DNA Library Prep Kit 2.0	12972ES	2.25 h	DNA Library for PE150
	Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit	12308ES	3.2 h	RNA library Prep
	Hieff NGS™ ds-cDNA Synthesis Kit	13488ES/ 13501ES	2.25 h	cDNA Library Prep
	Hieff NGS™ OnePot Flash DNA Library Prep Kit	12316ES	2.25 h	cDNA Library Prep
	Hieff NGS™ ds-cDNA Synthesis Kit+ Hieff NGS™OnePot Flash DNA Library Prep Kit	13488+12316ES	2.25 h	DNA&RNA co-Preparation library
	Hieff NGS™ 1st Strand Synthesis Kit	12946ES		tNGS
	4×Hieff™ Multiplex PCR Master Mix	12948ES	-
Multiplex PCR-based Amplicon Library Prep	Hieff™ Multiplex RT-PCR Kit	12950ES	-
	Hieff NGS™ Multiplex Long PCR Master Mix	17228ES	-	16S/18S/ITS Amp Master Mix
Bacterial 16S Detection	16S(V3-V4)Bacterial AmpSeq Prep Kit For Illumina	12983ES	-	16s rDNA(V3-V4)
	16S (V4) Bacterial AmpSeq Prep Kit for Illumina	12834ES	-	16s rDNA(V4)
	16S(V3-V4) Bacterial Metagenomic Library Construction Kit for MGI	12833ES	-	16s rDNA(V3-V4)
	16S (V4) Bacterial Metagenomic Library Construction Kit for MGI	12933ES	-	16s rDNA(V4)
Supporting Products	Hieff NGS™ One-Step rRNA Removal Kit （100-1000ng）	12258ES	17 min	mNGS RNA library
	Hieff NGS™ DNA Selection Beads	12601ES	-	DNA Clean up
	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set1/Set2/Set3/Set4（Plate）	12327-12330ES	-	Illumina UDI Adapter
	HieffNGS™ Unique Dual Barcode Primer Kit for MGI, Set1/Set2/Set3/Set4（Plate）	13350-13353ES		MGI UDB Adpter
	1×dsDNA HS Assay Kit /dsDNA HS Assay Kit /	12642ES	-	dsDNA Quantification
	ssDNA Assay Kit	12645ES	-	ssDNA Quantification
	Hieff NGS™ Fast-Pace DNA Cyclization Kit	13339ES	-	Cyclization Kit for MGI

tNGS Reagents for Pathogen Detection
Multiplex PCR	Cat:	Low DNA	Application
RT	12946	√	MMLV For Pathogen
RT-mPCR	12950	√	tNGS For Pathogen
mPCR	12948	√	NGS For Pathogen
mPCR	12977	√	NGS For Pathogen
mPCR	13283	√	Taq, 16S rDNA, Stool DNA
mPCR	17228	×	High fidelity DNA Pol, SNP, Stool DNA
mPCR-NGS Kit	12983	×	16s rDNA
Index Kit for Illumina	12416	√	index
Magnetic Beads	17266	√	Library DNA purification
Magnetic SpeedBeads	18397	√	Pathogen DNA purification

Adapter
Type	Product Name	Cat.No.	Note
Adapter For Illumina	Hieff NGS™ Complete Adapter Kit for Illumina, Set 1-Set 4(1-384 index)	12333ES-12336ES	Complete UDI Adapter for Illumina (576 Indexes, Plate Format）
	Hieff NGS™ Full UDI Adapter Kit for Illumina，Set5(385-480 index)	12356ES
	Hieff NGS™ Full UDI Adapter Kit for Illumina，Set5(481-576 index)	12357ES
	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set 1-Set 4(1-384 index)	12327-12330ES	Short UDI Adapter for Illumina (1536 Indexes, Plate Format)
	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set5~Set8(385-768 index)	12331ES
	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set9~Set12(769-1152 index)	12332ES
	Hieff NGS™ Stubby UDI Primer Kit for Illumina, Set13~Set16	12338ES
	Hieff NGS™ Tagment Index Kit for Illumina(96 Iindex)	17265ES	Tagment Index Kit (384 Indexes)
Adapter For MGI	Hieff NGS™ Complete Adapter Kit for MGI™, Set 1-Set 2	13361-13362ES	Single-Ended Complete Adapter for DNA (96 Indexes, Tube Format)
Adapter For MGI	Hieff NGS™ Unique Dual Barcode Primer Kit for MGI, Set 1-Set 4	13350-13353ES	Short UDI Adapter for Illumina(384 Indexes, Plate Format)

DNA/RNA Extration Kit
Spin Column Kit	Name	Cat.No.	Specification
RNA Extration	Hieff™ Cell/Tissue Total RNA Kit	19221	Tissue samples or cultured cells
	Hieff™ Plant RNA Kit	19291	Plant sample
	Hieff™ Plant Plus RNA Kit	19292	Plant samples of polysaccharides and polyphenols
	Hieff™ Micro Total RNA Kit	19212	Trace animal cells, microdissected tissues, and easily lysed animal tissues
	Hieff™ Bacterial RNA Kit	19301	Bacterial Sample
miRNA Extraction	Hieff™ Cell/Tissue miRNA Kit	19331	Tissues or cultured cells
miRNA Extraction	Hieff™ Serum/Plasma miRNA Kit	19332	Serum/Plasma
DNA Extraction	Hieff™ Cell/Tissue DNA Kit	18700	Tissues or cultured cells
	Hieff™ Blood/Cell/Tissue/Bacteria DNA fast Kit	18701	Blood/Cell/Tissue/Bacteria
	Hieff™ Fungal DNA Kit	18812	Fungal
	Hieff™ Soil DNA Kit	18815	Soil
	Hieff™ Magnetic Blood DNA Kit	18504	Blood/Cell
	Hieff™ Stool DNA Kit	18820	Stool
Plasmid Extraction	Hieff™ Endo-free Plasmid Mini Kit	19021	1-10 mL culture
	Hieff™ Endo-free Plasmid Midi Kit	19023	30-70 mL culture
	Hieff™ Endo-free Plasmid Maxi Kit V2	19037	150-300 mL culture
	Hieff™ Pro Endo-free Plasmid Maxi Kit	19038	50-200 mL culture
	Hieff™ Plasmid Mini Kit	19001
RNAsafe	RNAsafe Tissue Stabilizer	10604
RNase Remover	Surface RNase Remover (with Spray Bottle)	10609
Magnetic Beads	Hieff™ Magnetic SpeedBeads	18405
	Hieff™ Magnetic Universal Viral DNA/RNA Kit	18521
	Hieff™ Magnetic Tissue/Cell Total RNA Kit	18600
	Hieff™ Magnetic Pro Soil / Stool DNA Kit	18527