This kit is a total RNA library preparation kit for the Illumina and MGI sequencing platform, including RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific ds-cDNA synthesis reagents, and library amplification reagents. The sequencing library can be constructed followed by the mRNA purification kit(Cat#12629) or rRNA removal kit(Cat#122257 or Cat#12254). The two-strand synthesis module is equipped with two buffers to meet the need for routine library or strand-specific library. Among them, dTTP is replaced with dUTP in the strand-specific two-strand synthesis Buffer, so dUTP can be added to the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, achieving strand specificity. All reagents provided have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library preparation to the greatest extent.

Features

• Simplified process: two-strand synthesis, end repair and A addition are combined into one step.
• Wide compatibility: Compatible with strand-specific and non-strand-specific libraries: dNTP or dUTP amplification is used to achieve non-strand-specific or strand-specific library preparation. And compatible with Illumina and MGI libraries: primer mix for illumina or primer mix for MGI is used to achieve dual-platform compatibility.
• High-quality library preparation: Excellent sequencing data quality makes the coverage of transcript regions more uniform.

Specifications

Components

Workflow

Shipping and Storage

This product should be stored at -25~-15℃ for one years.

Application

RNA library preparation; LncRNA library preparation; M6A library preparation; transcriptome analysis; gene expression analysis; alternative splicing analysis; fusion gene detection;

Figures

1. RNA fragmentation condition selection

By optimizing the fragmentation system, the mRNA fragmentation effect of this kit is more concentrated and the effect is better. The fragmentation conditions are as follows:

94℃ 5min: 400~500bp;

94℃ 7min: 350~400bp;

94℃ 10min: 250~300bp.

Figure 1. Fragment size analysis under different RNA fragmentation conditions

Low-quality samples, high library preparation success rate

 Figure 2.  500 ng input of different DV200 Human FFPE RNA libraries.

*DV200 indicates the proportion of RNA fragments larger than 200 nt in the sample. For severely degraded FFPE samples, the DV200 value can better reflect the quality of the sample.

Conclusion:

Compared with other suppliers N*, yeasen RNA library preparation kit: High library preparation yield, high data utilization, low rRNA residual rate, and high exon ratio.

Chain-specific library preparation, high specificity

High consistency of multi-sample sequencing data

Figure 3. Consistency of library preparation and sequencing of samples with different starting amounts (samples are human RNA samples)

Stability test

Figure 4. Freeze-thaw stability(a) and room temperature stability testing(b)

Documents:

Safety Data Sheet

12308_MSDS_HB250529_EN.PDF

Manuals

12308_Manual_Ver.EN20250523.pdf

Citations & References:

[1] Deciphering the gut microbiome of grass carp through multi-omics approach
M Li, H Liang, H Yang, Q Ding, R Xia, J Chen, W Zhou… - Microbiome, 2024 IF:19.4
[2] Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition
L Fang, L Zhang, M Wang, Y He, J Yang, Z Huang… - Cancer Research, 2024 IF:12.7
[3] Klotho-derived peptide 1 inhibits cellular senescence in the fibrotic kidney by restoring Klotho expression via posttranscriptional regulation
X Zhang, L Li, H Tan, X Hong, Q Yuan, FF Hou… - Theranostics, 2024 IF:12.4
[4] Exoskeleton Partial‐Coated Stem Cells for Infarcted Myocardium Restoring
H He, Y Yuan, Y Wu, J Lu, X Yang, K Lu… - Advanced Materials, 2023 IF: 29.4
[5] Genomic innovation and regulatory rewiring during evolution of the cotton genus Gossypium
M Wang, J Li, Z Qi, Y Long, L Pei, X Huang… - Nature Genetics, 2022 IF:41.379
[6] MTMR3 risk alleles enhance Toll Like Receptor 9-induced IgA immunity in IgA nephropathy
Y Wang, T Gan, S Qu, L Xu, Y Hu, L Liu, S Shi, J Lv… - Kidney International, 2023 IF:19.6
[7] Split complementation of base editors to minimize off-target edits
X Xiong, K Liu, Z Li, FN Xia, XM Ruan, X He, JF Li - Nature Plants, 2023 IF:18.6
[8] Engineered bacterial outer membrane vesicles encapsulating oncolytic adenoviruses enhance the efficacy of cancer virotherapy by augmenting tumor cell autophagy
W Ban, M Sun, H Huang, W Huang, S Pan… - Nature Communications, 2023 IF:16.6
[9] Cancer Cell Resistance to IFNγ Can Occur via Enhanced Double-Strand Break Repair Pathway Activity
T Han, X Wang, S Shi, W Zhang, J Wang, Q Wu… - Cancer Immunology Research, 2023 IF: 12.0
[10] Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma
Y Huang, Q Kou, Y Su, L Lu, X Li, H Jiang… - Journal of Nanobiotechnology, 2023 IF: 10.9
[11] PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma
W Bao, J Wang, K Fan, Y Gao, J Chen - Pharmacological Research, 2023 IF:9.3
[12] Identification of RNA-Binding Protein Targets with HyperTRIBE in Saccharomyces cerevisiae
W Piao, C Li, P Sun, M Yang, Y Ding, W Song… - International Journal of molecular Science, 2023 IF:5.6
[13] Microbiota regulates life-cycle transition and nematocyte dynamics in jellyfish
S Peng, L Ye, Y Li, F Wang, T Sun, L Wang, W Hao… - Iscience, 2023 IF: 5.08
[14] Transcriptome and miRNAs Profiles Reveal Regulatory Network and Key Regulators of Secondary Xylem Formation in “84K” Poplar
H Wang, P Zhao, Y He, Y Su, X Zhou… - International Journal of molecular Science, 2023 IF:5.6