Depending on the nature of the sample, NGS library construction can be categorized into DNA library construction and RNA library construction.The DNA library preparation has five standard molecular steps including DNA Fragmentation, End repair, dA-Tailing, Adapter ligation and PCR amplification. The RNA library preparation has additional reverse transcription and secondary strands synthesis steps. Enzymes play a pivotal role in these interconnected experiments. So, which key enzymes are involved in the process of library construction?
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Hieff™ Versatile T4 DNA Ligase (600 U/µL)- Thermotolerant and High Fidelity 12996ES
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Single-Stranded DNA Binding Protein, SSB (Thermostable, 5μg/μL) _ 14561ES
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2×Super Canace™ II High-Fidelity Mix for Library Amplification _12621ES
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