Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of a template and a primer. This enzyme has a 3'→5' exonuclease activity, but it does not have a 5'→3' exonuclease function.
Applications
3´ overhang removal to form blunt ends
5´ overhang fill-in to form blunt ends
Second strand synthesis in site-directed mutagenesis
Probe labeling using replacement synthesis
Cloning of PCR products without reliance on ligation reactions
Specifications
|
Source |
Bacteriophage T4 |
|
Molecular Weight |
103 kDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide triphosphates (dNTPs) into a polynucleotide within 30 minutes at 37°C |
Components
|
Name |
12901ES60 |
12901ES76 |
12901ES90 |
12901ES08 |
|
|
12901-A |
T4 DNA Polymerase(5 U/μL) |
20 μL |
100 μL |
400 μL |
1 mL |
|
12901-B |
10 × Blue Buffer |
100 μL |
250 μL |
500 μL |
1.25 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
Payment & Security
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