A "strand-specific" library is constructed by PCR amplification using only one strand of cDNA reverse-transcribed from RNA as the template. In contrast, a conventional transcriptome library uses both strands of cDNA for PCR amplification. As a result, a conventional transcriptome library cannot distinguish RNA polarity, while a "strand-specific" library can effectively distinguish RNA polarity. This is the key difference between the two types of libraries.
Advantages:
- More accurate quantification of transcript expression levels.
- More accurate detection of alternative splicing events.
- Increased detection rate of non-coding transcripts and discovery of potential cis-natural antisense transcripts.
- Better prediction of prokaryotic operons.
- More efficient and accurate transcript assembly.
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Yeasen
Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific)-12340ES
Sale priceFrom $695.00
Regular price$925.00
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Yeasen
Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit -12308ES
Sale priceFrom $495.00
Regular price$695.00