Hieff NGS^TM EvoMax RNA Library Prep Kit (Strand-specific) is a premixed, actinomycin D–free, strand-specific total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. Available in two formats—tube and sealing kit—this kit offers convenience for both automated liquid handling systems and manual operation.

The kit includes reagents for RNA fragmentation, reverse transcription, strand-specific double-stranded cDNA synthesis, and library amplification. It can be combined with mRNA purification or rRNA removal kits for mRNA or lncRNA studies.

By optimizing the reverse transcription step, this kit achieves high strand specificity without the use of actinomycin D, enhancing user safety. All reagents undergo stringent quality control and functional validation to ensure maximum stability and reproducibility in library preparation.

Features

• Premixed Reagents: Enzymes and buffers are pre-mixed to reduce reagent components and simplify preparation.
• Safe and Light-Resistant with Improved Strand Specificity: Optimized reverse transcriptase ensures high strand specificity without the need for light protection during handling.
• Preloaded Plate Format—Ready to Use: Designed for seamless integration with automation instruments, the preloaded plates enable easy “tear-and-use” operation for convenient automated workflows.

Components

[Note]: * The notation indicates that the component is not included in the kit. Primer mix is required if the complete adapters are used, otherwise it isn’t. This kit is compatible with Illumina and MGI platforms, but need additional primer mix (Cat # 13334 Primer Mix for MGI and Cat # 13335 Primer Mix for Illumina) for Illumina or MGI platform if the complete adapters are used.

** See the following diagram for the layout of plate reagent group.

Storage

This product should be stored at -25~-15℃ for one year..

Application

RNA library Preparation; Transcriptome profiling and gene expression analysis; mRNA sequencing for coding RNA studies; lncRNA (long non-coding RNA) research; Small RNA sequencing (if applicable); Differential gene expression analysis; RNA biomarker discovery; RNA variant and fusion detection.

Figures

1.Simplified Product Format

Figure 1. Simplied components of EvoMax RNA Library Prep Kit.

2.Flexible RNA Library preparation: High and Low Input Compatible

Figure 2. Library yields of animal and plant samples with different RNA qualities and input amounts.

Library preparation with kits(Cat# 12629) and 12341; DNA fragmentation at 94°C for 7 min; PE Adapter (Cat#12327ES), 1 μM, 5 μl; 0.4× purification after ligation, 0.7×/0.1× size selection, and 0.8× purification post-amplification. Input amounts: 100 ng (16 cycles), 200 ng (15 cycles), 400 ng (14 cycles).”

3. Automation-friendly

Figure 3. Highly uniform mRNA and lncRNA libraries prepared with 96-channel automation.

4. Excellent Sequencing Data Performance

Figure 5. Performance of transcriptome sequencing using EvoMax RNA Library Prep Kit

The transcriptome sequencing data shows good performance in gene body coverage, insert DNA length distribution, and sample correlation

5.Strong Stability

Figure 6. Real-time stability: After 18 months of storage, performance remains comparable to the reference kit.

6.RNA Library prep for different quality FFPE samples

For severely degraded FFPE RNA (DV 200 <50%) and low input samples, we recommend a Double-purification protocol after adapter ligation to reduce library loss.

Peak patterns of different quality FFPE samples

Documents:

Safety Data Sheet

12340_MSDS_HB250620_EN.PDF

Manuals

12340_Manual_Ver.EN20250706.pdf

Related Blog:

A New Era of RNA Library Prep: The World’s First Pre-mixed Kit Without Toxic Actinomycin D