Description
Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxynucleotides to the 3'-hydroxyl termini of oligonucleotides, single-stranded, and double-stranded DNA. The TdT reaction requires short sequences of at least three bases to act as primers. When using RNA as a substrate, TdT activity is highly dependent on the tertiary structure and nucleotide composition at the 3’-end of the acceptor RNA. In general, TdT exhibits lower efficiency with RNA templates compared to DNA templates.
Specification
|
Cat. No. |
10302ES76 / 10302ES86 / 10302ES99 / 10302ES98 |
|
Size |
500 U / 2,500 U / 180,000 U / 333,000 U |
|
Concentration |
20 U/µL |
|
Source |
Recombinant expression in E.coli |
|
Unit Definition |
One unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of deoxyribonucleotide into polydeoxynucleotides in 60 minutes at 37°C. |
|
Inhibitors |
Metal chelators, ammonium, chloride, iodide, and phosphate ions. |
|
Inactivation |
Add EDTA and heat at 70°C for 10 min. |
Components
|
Components No. |
Name |
10302ES76 |
10302ES86 |
10302ES99 |
10302ES98 |
|
10302-A |
Terminal Deoxynucleotidyl Transferase (20 U/µL) |
25 μL |
125 μL |
9 mL |
16.65 mL |
|
10302-B |
5× Reaction Buffer |
400 μL |
2×1 mL |
40 mL |
74 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Application
DNA End Labeling; TUNEL Assay for Apoptosis Detection; Next-Generation Sequencing (NGS) Library Preparation; DNA Tail Extension and Modification; Molecular Diagnostics and Biosensing(CRISPR or DNAzyme biosensors).
Figures
1. High purity — no nicking enzyme detectable, no endonuclease detected.
Figure 1. Residual nicking enzyme and exonuclease activities were tested in two independent batches, and no residues were detected.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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