1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.

Features

• Fast: Ready-to-use premixed solution for easy handling; results available in just 5 minutes.
• Sensitive: Accurately quantifies samples ranging from 0.2 to 100 ng.
• Accurate: Minimal deviation compared to competing products, with all errors controlled within 10% and low batch-to-batch variation.
• Stable: Consistent performance across different production batches.

Components

Shipping and Storage

This product should be stored at 2~8℃ away from light for 12 months.

Application

dsDNA quantification; DNA concentration determination

Figures

1. Easy-to-use and streamlined quantification workflow

 Figure 1. DNA Quantification Workflow

2. Good linearity:  12642 has good linearity in the range of 0.2-100 ng.

 Figure 2. Linear Relationship Test

Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12642. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.

3. Dye binding specificity

 Figure 3. Specificity test

Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.

Conclusion: Adding equal amounts of ssDNA slightly increases the 12642 reading; error <10%(left). Adding equal amounts of RNA also increases the 12642 reading; error <10%(right).

4. Efficient dye binding and stable fluorescence signal

 Figure 4. Dye binding efficiency and fluorescence signal persistence

Test Scheme: Two RNA libraries of different concentrations were measured using 12642 on the Qubit 3.0 with a 2 μL sample volume.

Conclusion: The fluorescence signal saturated within 2 minutes and remained stable for up to 5 h.

5.  Strong tolerance to common contaminants

6. Ultra-high stability

 Figure 5. Stability comparison of Yeasen's  (Cat#12642ES) and competitor T brand

Test Scheme: Two dsDNA concentrations were measured over time using supplier T* (4°C), Yeasen#12642 (4°C), and Yeasen#12642 (room temperature, ~25°C). Deviation from the initial value (Day 0) was calculated.

Conclusion: Yeasen#12642 remained stable at room temperature for 2 weeks, with deviations <10%.

FAQ

Q: What is the difference between 12640 and 12642?

A: 12642 is a premixed solution, ready-to-use. It can be directly added to the sample to determine the concentration. There is no need to prepare a dye working solution.

Q: The product composition consists of only two standard substances. When creating the standard curve, is it necessary to perform multiple dilutions on the "standard 2" sample?

A: When using Qubit for quantification, only two standards are needed according to the operation steps. If using an enzyme reader, multiple gradients are required to create the standard curve. In this case, 1×TE can be used to gradientally dilute standard 2.

Q: If we replace Qubit detection with an enzyme detector, how should we select the enzyme detection plate and how should the wavelength be set?

A: Select the black microplate. Wavelength: Ex = 480 nm, Em = 520 nm.

Q: Is there a difference between the fluorescence value of the standard sample and the previous ones? What should the typical fluorescence value of a standard sample be?

A: The fluorescence value of the standard sample is not fixed, and there may be differences among different instruments. To verify, you can perform multiple replicate wells or use multiple gradients, and if a linear relationship is observed, it indicates success.

Documents:

Safety Data Sheet

12642_MSDS_HB250619.PDF

Manuals

12642_Manual_Ver.EN20250619.pdf