1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.

Features

• Fast: Ready-to-use premixed solution for easy handling; results available in just 5 minutes.
• Sensitive: Accurately quantifies samples ranging from 0.2 to 100 ng.
• Accurate: Minimal deviation compared to competing products, with all errors controlled within 10% and low batch-to-batch variation.
• Stable: Consistent performance across different production batches.

Components

Shipping and Storage

This product should be stored at 2~8℃ away from light for 12 months.

Application

dsDNA quantification; DNA concentration determination

Figures

1. Easy-to-use and streamlined quantification workflow

 Figure 1. DNA Quantification Workflow

2. Good linearity:  12642 has good linearity in the range of 0.2-100 ng.

 Figure 2. Linear Relationship Test

Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12642. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.

3. Dye binding specificity

 Figure 3. Specificity test

Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.

Conclusion: Adding equal amounts of ssDNA slightly increases the 12642 reading; error <10%(left). Adding equal amounts of RNA also increases the 12642 reading; error <10%(right).

4. Efficient dye binding and stable fluorescence signal

 Figure 4. Dye binding efficiency and fluorescence signal persistence

Test Scheme: Two RNA libraries of different concentrations were measured using 12642 on the Qubit 3.0 with a 2 μL sample volume.

Conclusion: The fluorescence signal saturated within 2 minutes and remained stable for up to 5 h.

5.  Strong tolerance to common contaminants

6. Ultra-high stability

 Figure 5. Stability comparison of Yeasen's  (Cat#12642ES) and competitor T brand

Test Scheme: Two dsDNA concentrations were measured over time using supplier T* (4°C), Yeasen#12642 (4°C), and Yeasen#12642 (room temperature, ~25°C). Deviation from the initial value (Day 0) was calculated.

Conclusion: Yeasen#12642 remained stable at room temperature for 2 weeks, with deviations <10%.

Documents:

Safety Data Sheet

12642_MSDS_HB250619.PDF

Manuals

12642_Manual_Ver.EN20250619.pdf