Hieff NGS^TM One Pot Flash DNA Library PrepKit is a rapid enzymatic DNA library kit，witch contains high quality enzyme for DNA fragmentation and combines DNA fragmentation, end-repair, and dA-tailing into one step, which reduce time significantly and cost of library preparation.

This library prep kit compatible with 100 pg-500 ng samples of all common animals, plants, microorganisms, etc. And quickly realize DNA fragmentation, terminal repair and A-tail addition reaction.The kit needs to be matched with adapters and primers, and is compatible with Illumina^TM and MGI^TM high-throughput sequencing platforms.

Features

• Time-saving: Ultra-fast library preparation in under 2 hours—single-tube fragmentation, end repair, and A-tailing completed in just 10 minutes.
• Labor-saving: Simple one-tube enzymatic reaction for efficient DNA fragmentation.
• Sample-saving: Compatible with input DNA as low as 100 pg.
• Low DNA residue: Strictly control the residue of host nucleic acid

Components

Storage

This product should be stored at -25~-15℃ for one year..

Application

DNA library Preparation; Metagenomic sequencing(mNGS); Microbial genome sequencing.

Figures

1.Ultra-Rapid Library Prep Workflow

Figure 1. The workflow of One Pot Flash DNA Library Prep Kit

2.Extensive sample compatibility

Figure 2. Rapid DNA Library Preparation with Broad Sample Type Compatibility.Compatible with gDNA, cDNA, PCR Products, and cfDNA, delivering library yields >1 μg.

3.Robust Library Yields Over a Broad DNA Input Range

Figure 3. Rapid DNA Library Preparation with Broad DNA/cDNA Input Compatibility

(A) Left: Libraries were prepared using the Flash OnePot DNA Library Prep Kit (Cat#12316) with 100 pg to 500 ng of ZymoBIOMICS Microbial DNA Standards(Cat# D6306), showing excellent performance across input amounts.

(B) Right: The kit is compatible with unpurified cDNA inputs from 10 ng to 500 ng.

4. Excellent Sequencing Data Performance

Figure 4. Performance of mNGS Data on insert DNA and Microbial detection

Libraries were prepared from 10 ng of ZymoBIOMICS Microbial DNA Standard (Cat# D6306) using the Flash OnePot DNA Library Kit (Cat#12316). Libraries were pooled and sequenced on an Illumina MiSeq (2 ×150 bp). The detected microbial composition closely matched the expected proportions. Expected composition: Cryptococcus neoformans 2%, Saccharomyces cerevisiae 2%, Bacillus subtilis 12%, Escherichia coli 12%, Enterococcus faecalis 12%, Lactobacillus fermentum 12%, Listeria monocytogenes 12%, Pseudomonas aeruginosa 12%, Staphylococcus aureus 12%, and Salmonella enterica 12%.

Documents:

Safety Data Sheet

12316_MSDS_HB250706_EN.PDF

Manuals

12316_Manual_Ver.EN20250706.pdf