Hieff NGS^TM DNA Library Prep Kit is a new generation library preparation kit specially developed and designed for Illumina&MGI sequencing platform. On the basis of the previous generation of library preparation kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.

Features

• Broad Sample Compatibility: Supports a wide range of input DNA from 100 pg to 1 µg, covering all common sample types.
• High Adapter Ligation Efficiency: Powered by a novel ligase mutant to minimize adapter dimers and maximize library conversion rates.
• Accurate Mutation Detection: Incorporates a next-generation high-fidelity enzyme to enhance the precision of low-frequency variant detection.
• Excellent Reagent Stability: Single-use library prep reagents remain stable for up to 4 weeks at 4°C and 2 weeks at 25°C, fully compatible with automated workflows

Components

Shipping and Storage

This product should be stored at -25~-15℃ for 1 year..

Application

DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing;

Figures

1. Multi-Species Compatibility

Figure 1. Library Yield Comparison Across Species

Library preparation was performed using 50 ng of genomic DNA from different species. Yeasen’s 12927ES (2G) kit produced equal or higher library yields than 13577ES (1G), and showed superior performance compared to supplier N* and K*.

2. Compatible with Illumina and MGI platforms

Figure 2. Compatibility of UDI Adapters and complete adapter with Illumina and MGI Platforms

3. Excellent Sequencing Data Performance

Figure 3. Comparison of Sequencing Data Quality

Using tumor SNV and wild-type gDNA/ctDNA/FFPE DNA standards with 50 ng/10 ng input and 6-plex hybrid capture (Cancer SLC Panel), Kit 12927 showed lower mismatch, insertion, and deletion rates, indicating higher data accuracy. 

4. Stability

Figure 4. Stability evaluation of the library preparation kit under different storage conditions.

Library yield remained consistent with the control after storage at 4 °C and 25 °C for 1 to 4 weeks, indicating good reagent stability under short-term elevated temperatures.

Documents:

Safety Data Sheet

12927_MSDS_HB250620_EN.PDF

Manuals

12927_Manual_Ver.EN20250618.pdf