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mRNA IVTSolution (Two-Step Enzymatic Capping)

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Application	Category	Cat.NO.	Name	Note
Template Preparation	Restriction endonucleases	10664	BspQI GMP-grade (10 U/μL)
		10667	10×Digestion buffer 3 GMP-grade1
		10661	Bsa I GMP-grade (20 U/μL)
		10668	10×Digestion buffer 4 GMP-grade
		15033	FuniCut™ Xbal
	Cloning	10923	Hieff Clone™ Universal II One Step Cloning Kit
	T4 DNA ligase	10300	Hieff™ Gold T4 DNA Ligase(5 U/µL)
	Plasmid extraction	19037	Hieff™ Endo-free Plasmid Maxi Kit V2
	High-fidelity PCR	10166	Hieff Canace™ Veritas High Fidelity Long PCR Master Mix (With Dye)
In Vitro Transcription Kit	In Vitro Transcription Kit	10623	T7 High Yield RNA Synthesis Kit	Conventional T7 RNAp
In Vitro Transcription Kit	In Vitro Transcription Kit	10633	T7 High Yield RNA Synthesis Kit (low dsRNA)	Low dsRNA T7 RNAp
In vitro transcription (IVT) Module	T7 RNA Polymerase	10628	CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)	Low dsRNA T7 RNAp
		10625	T7 RNA Polymerase GMP-grade (250 U/μL)
		10629	Hieff™ T7 RNA Polymerase GMP-grade (low dsRNA, 250 U/μL)
	10X Transcription Buffer	10670	10× Transcription Buffer 2 GMP-grade
	10X Transcription Buffer	10669	10×Transcription Buffer GMP-grade (Mg2+ free)
	RNase Inhibitor	10621	Murine RNase inhibitor GMP-grade (40 U/μL)
	Inorganic Pyrophosphatase	10620	Pyrophosphatase, Inorganic GMP-grade (1 U/μL)
		10658	Pyrophosphatase,Inorganic (1 U/μL) (IPPA)
		10672	Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
	NTP(Tris)	10652	ATP Tris Solution GMP-grade (100 mM)
		10653	CTP Tris Solution GMP-grade (100 mM)
		10654	UTP Tris Solution GMP-grade (100 mM)
		10655	GTP Tris Solution GMP-grade (100 mM)
	Pseudouridine	10656	Pseudo UTP Tris Solution GMP-grade (100 mM)
	N1-methylpseudouridine	10657	N1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM)
	NTP(Na）	10129	ATP Solution(100 mM) (100 mM)
		10130	CTP Solution(100 mM) (100 mM)
		10131	UTP Solution(100 mM) (100 mM)
		10132	GTP Solution(100 mM) (100 mM)
	Premix	10133	NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each）
	Pseudouridine triphosphate sodium salt	10650	Pseudouridine-5-triphosphate,trisodium salt solution (100 mM)
	N1-methylpseudouridine triphosphate sodium salt	10651	N1-Me-Pseudo UTP sodium solution(100 mM)
Nuclease Digestion	DNase I	10611	UCF.ME Deoxyribonuclease I (DNase I) GMP-grade
	RNase R	14616	10×RNase R Reaction Buffer GMP-grade	For cicrRNA application
		14615	RNase R GMP-grade (20 U/μL)	For cicrRNA application
		14606	RNase R (20 U/μL)	For cicrRNA application
Enzymatic Capping	SAM	10619	S-adenosylmethionine (SAM) GMP-grade (32 mM)	Two-step Capping (Single-component)
	Vaccinia Capping Enzyme	10615	mRNA Vaccinia Capping Enzyme (10 U/μL)
	Vaccinia Capping Enzyme	10614	mRNA Vaccinia Capping Enzyme GMP-grade (10 U/μL)
	2'-O-Methyltransferase	10616	mRNA Cap 2´-O-Methyltransferase (50 U/μL)
	2'-O-Methyltransferase	10612	mRNA Cap 2'-O-Methyltransferase GMP-grade (50 U/μL)
	10X Capping Buffer	10666	10×Capping Buffer GMP-grade
	GTP Substrate (for capping)	10655	GTP Tris Solution GMP-grade (100 mM)
		17121	Vaccinia Capping System	Two-step Capping Kit
mRNA Quality Control		36705	T7 RNA Polymerase ELISA kit
		36709	Vaccinia Capping Enzyme ELISA Kit
		36707	Murine RNase Inhibitor ELISA Kit
		41309	RNase Viability Assay Kit (Fluorescent Labeling)
		36721	E.coli HCP ELISA kit
		41308	E.coli Host Cell DNA Residue Detection Kit (2G)
Poly(A) tailing		14801	E.coli poly(A) Polymerase (5 U/μL)
mRNA Purification	Magnetic bead-based purification	12602	Hieff NGS™ RNA Cleaner
mRNA Purification	Lithium chloride precipitation method

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mRNA IVTSolution (Two-Step Enzymatic Capping)

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