Description
This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.
Features
- DA DMF Certified** (MF038306)
- GMP Grade, Animal-Origin Free: Manufactured under ISO 13485 and GMP standards
- High Efficiency, Low Star Activity: Full digestion at 37°C in 16 hours, no star activity
- Nuclease-Free: Rigorously tested and confirmed free of endonucleases, exonucleases, and RNases to ensure sample integrity and reliable downstream results
- Performance Matches Leading Brands
Application
- Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
- Linearize plasmid template before in vitro transcription
- Restriction digest
Specification
| Expression Host | Recombinant E. coli with BspQI gene |
| Reaction Temperature | 50℃ |
| Storage Buffer | 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol |
| Unit Definition | 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system |
Components
| Components No. | Name | 10664ES76 (500 U) | 10664ES86 (2,500 U) | 10664ES92 (10 KU) | 10664ES98 (100 KU) |
| 10664 | BspQI GMP-grade (10 U/μL) | 50 μL | 250 μL | 1 mL | 10 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
1. Enhanced Restriction Enzyme Digestion Efficiency
Figure 1. Comparison of Digestion Efficiency at Different Enzyme Amounts (0.125–4 U): 2 U Delivers Optimal Performance
In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M: DNA marker
C: The control group without BspQI treatment
2. High-Efficiency Enzymatic Digestion in 0.5–3 Hours
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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