Description
This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.
Feature
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Restriction Enzyme Cut Site: GCTCTTC (1/4)
- Digestion of DNA to obtain specific sticky ends
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, RNases
Application
- Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
- Linearize plasmid template before in vitro transcription
- Restriction digest
Specification
Expression Host | Recombinant E. coli with BspQI gene |
Reaction Temperature | 50℃ |
Storage Buffer | 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol |
Unit Definition | 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system |
Components
Components No. | Name | 10664ES76 (500 U) | 10664ES86 (2,500 U) | 10664ES92 (10 KU) | 10664ES98 (100 KU) |
10664 | BspQI GMP-grade (10 U/μL) | 50 μL | 250 μL | 1 mL | 10 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figure1. The performance of YEASEN BspQI is superior.
In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M: DNA marker
C: The control group without BspQI treatment