Murine RNase inhibitor is a specific inhibitor of ribonuclease (RNase) found in the human placenta, which specifically inactivates RNase by binding to it non-covalently to form a complex that inactivates RNase.

This kit uses the principle of double-antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residual Murine RNase Inhibitor in samples. First, add the Murine RNase Inhibitor standard and test sample to the Anti-Murine RNase Inhibitor coated microtiter strips (36707-A), then add the diluted biotin labeled Anti-Murine RNase Inhibitor antibody (36707-C), and finally add Streptavidin-HRP (36707-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. Subsequently, add TMB substrate (36707-H) into the complex to observe color reaction after washing the complex. TMB is converted into blue under the catalysis of HRP enzyme and finally converted into yellow in the presence of acid, and the shade of color is positively correlated with the amount of Murine RNase Inhibitor in the sample.

The detection range of this kit is 2~128 ng/mL; the lower detection limit is 1.5 ng/mL.

Feature

• Highly sensitive: Detecting as little as 1.5 ng/mL of Murine RNase Inhibitor residuals in in-process and final biological samples.
• Ensures accuracy: The Murine RNase Inhibitor residuals can be accurately detected with a recovery of 75%-125%.
• Specificity: Cooperate use with YEASEN Murine RNase Inhibitor and can accurately detect the residuals.
• Stable: The difference between lot-to-lot is low, the kit property is not impacted under 37℃ for 7 days.
• Easy signal collection: Using Biotin-Streptavidin system, the signal can be stably enlarged.

Application

• Residual Murine RNase Inhibitor test in biological products
• Residual Protein Detection in mRNA Vaccine and Therapeutic Production;
• Purification Validation in Nucleic Acid Synthesis and In Vitro Transcription (IVT)

Specification

Components

^*The Standard (36707-B) is Lyophilized powder.

Storage

This product should be stored at 2°C ~8°C. Unopened product is valid for one year.

^*Upon receipt of the kit, please check whether all components are complete and immediately store them in corresponding condition.

Figures

• Precision 

A) Repeatability

B) Intermediate precision

Table 1. The precision of Murine RNase Inhibitor ELISA was assayed as the above scheme

10 duplicated were tested at 3 concentrations on the standard curve, and all the CV% were lower than 10% (Table 1A). 3 lot of Salt Active UltraNuclease ELISA kits were tested and the CV% were lower than 15% (Table 1B).

• Accuracy 

Three concentrations of Murine RNase Inhibitor standards, high, medium and low, were added to the mRNA purified samples in equal proportions, while the high value spiked samples were made multiplicative dilutions.

Spiked recovery = measured value / (spiked standard x 50% + spiked specimen value x 50%)

Table 2. Accuracy Validation

The linear and spiked recoveries of Murine RNase Inhibitor in the doubly diluted samples, as well as the samples with different spiked concentrations, and in the unspiked samples, ranged from 80% to 125% (Table 2).

Documents:

Safety Data Sheet

36707_MSDS_HB250226.pdf

Manuals

36707_Manual_Ver.EN20250226.pdf