Description
This product is an optimized transcription buffer and is efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents, and magnesium ions required for enzyme proteins.
This product is produced by GMP regulations and provided in liquid form.
Application
- RNA in vitro transcription
Components
| Components No. | Name | 10670ES03 | 10670ES10 | 10670ES25 | 10670ES76 |
| 10670 | 10× Transcription Buffer 2 GMP-grade | 1 mL | 10 mL | 25 mL | 500 mL |
Storage
This product could be stored at -25℃ ~ -15℃ for one year.
Notes
For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
FAQ
Q: The transcription buffer of Yisheng has a Mg2+ concentration of 460 mM, which is higher than that of N*, T*, and other domestic manufacturers. Relevant literature reports that the Mg2+ concentration has a significant impact on the amount of transcriptional by-products, dsRNA. Are there any relevant research data?
A: The concentration of our transcribed Buffer is 10X, and the final concentration in the IVT system is 46 mM, which is higher than that of some other brands. We have some data from a single-factor study on Mg2+ (the details are not convenient to disclose). We have determined this concentration. We also provide a buffer without Mg2+ (10669ES) to facilitate customers to adjust the final concentration of Mg2+ in the transcription system by themselves, thereby regulating the balance between IVT yield and by-products.
Q: Are there any differences between 10670ES and 10627ES?
A: The formulas are different. The 10670ES is an upgraded version. We highly recommend using the 10670ES. It has a wider application scope and better performance.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.