N1-Me-Pseudo UTP solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and changes the innate immune receptor and in vitro transcription. The interaction of RNA has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Features

• Validated, product-specific manufacturing processes and analytical methods compliant with ISO 13485 and GMP requirements.
• Product-specific stability studies ensuring consistent performance. AOF (Animal-Origin Free) production process and raw materials, with complete TSE/BSE statements.
• Nitrosamine risk assessment and statement available.
• Comprehensive regulatory support documents provided.
• Scalable manufacturing capacity for large-scale production.
• Multiple salt forms available (Na⁺, Tris, etc.) to meet diverse downstream application needs.
• Optimized Tris–NTP reaction system delivering increased IVT yield and reduced dsRNA content.

Application

• RNA synthesis and amplification
• Building block for in vitro transcription

Specification

CAS No.	1428903-59-6 (free acid)
Molecular formula	C10H17N2O15P3 (free acid)
Molecular weight	498.17 g/moL (free acid)
Purity	≥ 99%
Content	100mM ± 3mM
Structure

Component

Storage

The product should be stored at -25℃ ~ -15℃ for two years.

Figures

• Increased the IVT yield

Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.

• Decreased the dsRNA content

Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by the Dot Blot method.