The Hieff™ Plant Plus RNA Kit adopts Hieff™ RNA Column and a new solution system, which is suitable for extracting high-purity and high-quality total RNA from plant tissue samples rich in polysaccharides and polyphenols. The operation is simple, and the total RNA can be extracted in 30 min. The DNA-Removing Column in the kit can easily adsorb and remove genomic DNA, while the Hieff™ RNA Column can efficiently bind RNA, and the extracted total RNA has high purity, which can be used in various molecular biology experiments such as RT-PCR, Real-Time PCR, molecular cloning and RNase protection analysis.
Features
Safety: no need to use organic reagents such as phenol chloroform.
Fast: RNA extraction from samples can be completed within 30 min minutes.
High RNA purity: DNase I column digestion can effectively remove gDNA.
Wide compatibility: It is suitable for RNA extraction from rice, corn, wheat, Arabidopsis, tomato, tobacco and general polysaccharides and polyphenols such as cotton and holly.
Applications
Plant RNA extraction; Total RNA extraction from plants; RNA Extraction from Polysaccharide Polyphenols Plants
Specifications
|
Cat.No. |
19292ES08 /19292ES50 |
|
Size |
5 T / 50 T |
Components
|
Components No. |
Name |
19292ES08 （5T) |
19292ES50 (50T) |
|
19292-A |
RNA Adsorption Column P5 (HieffTM RNA Column P5) |
5 |
50 |
|
19292-B |
2 mL Collection Tube P5 |
10 |
100 |
|
19292-C |
DNA-Removing Column P5 |
5 |
50 |
|
19292-D |
Lysate LB (LB Buffer P5) |
2.5 mL |
25 mL |
|
19292-E |
Helper Buffer B5(HB Buffer B5) |
600 μL |
6 mL |
|
19292-F |
Binding Buffer BD*(BD Buffer P5) |
2.4 mL |
24 mL |
|
19292-G |
Deproteinized solution PL (PL Buffer P5) |
2.5 mL |
25 mL |
|
19292-H |
Wash Buffer W* |
2.4 mL |
24 mL |
|
19292-I |
RNase-free H2O |
1 mL |
10 mL |
Shipping and Storage
Transport and store at room temperature.The product is valid for two years.
Figures
Table 1. High yield and purity of 19292 extracted products
|
Suppliers |
Concentration(ng/μL) |
A260/A280 |
A260/A230 |
|
YEASEN-19292 |
42.805 |
1.802 |
1.731 |
|
YEASEN-19292 |
44.836 |
1.769 |
1.672 |
|
T-1 |
29.17 |
1.674 |
1.860 |
|
T-2 |
33.89 |
1.622 |
1.748 |
|
O-1 |
10.795 |
1.789 |
0.717 |
|
O-2 |
11.533 |
1.592 |
0.445 |
【Note】：The leaves of Prunus mume, 50 mg, were extracted with 19292 and products from O supplier and T supplier, and eluted with 50 ul ddH2O.
FAQ
Q: Have your plant DNA extraction kit been tested for samples like olive leaves?
A: If you haven't tested the olive leaves, you can try the polysaccharide and polyphenol extraction kit.
Documents:
Safety Data Sheet
Manuals
Citations & References:
Related blog：
Storage
FAQ
Citations
Documents
Applications
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