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This kit is designed for the isolation and purification of high-quality total RNA from a wide variety of sample types, including animal tissues (heart, liver, spleen, lung, muscle, fat, brain, etc.), cells, common plants, and polysaccharide‑ and polyphenol‑rich plant samples. A unique magnetic bead system combined with a carefully optimized buffer formulation efficiently captures the released nucleic acids, yielding RNA of high purity with minimal carry‑over of genomic DNA, protein and other impurities. The purified RNA is suitable for a broad range of downstream applications such as RT-PCR, real-time PCR, microarray analysis, Northern blot, dot blot, poly(A) selection, in vitro translation, RNase protection assay, and molecular cloning. The kit is fully compatible with YEASEN automated nucleic acid extraction instruments and the Thermo Scientific KingFisher Flex 96 and comparable systems. YEASEN offers integrated solutions for high‑throughput automated nucleic acid extraction.

Features

Broad sample compatibility: Works with various animal and plant tissues, including liver, kidney, spleen, heart, skin, aquatic samples, leaves, roots, fruits, and seeds.

Fast and simple: Extraction takes less than 1 hour per sample. Fully compatible with Yeasen 16/48/96-channel extraction instruments.

High-purity RNA: Includes DNase I for complete DNA removal. Ready for RT-PCR, library prep, and other downstream applications.

Components

Category	No.	Name	18613ES48
Part I	18613-A	DNase I	0.6 mL/tube × 1
Part II	18613-B	Proteinase K	1.1 mL/tube × 1
Part III	18613-C	Lysis Buffer AL	16 mL/bottle × 1
	18613-D	Lysis Buffer PL	50 mL/bottle × 1
	18613-E	Digestion Buffer DR	40 mL/bottle × 1
	18613-F	Binding Buffer RB	2 mL/bottle × 1. Add 28 mL isopropanol prior to first‑time use
	18613-G	Magnetic Bead Suspension	1.1 mL/tube × 1
	18613-H	Wash Buffer DW1	19 mL/bottle × 1. Add 19 mL absolute ethanol prior to first‑time use
	18613-I	Wash Buffer DW2	18 mL/bottle × 1. Add 72 mL absolute ethanol prior to first‑time use
	18613-J	Elution Buffer RE	5 mL/bottle × 1

Notes

1. If precipitation or turbidity appears in any reagent (common when stored at low ambient temperatures), warm to 30 °C and mix until fully dissolved.

2. Magnetic beads may carry over into the eluate. Avoid aspirating magnetic beads when transferring eluted RNA.

3. Avoid repeated freeze-thaw cycles of frozen samples, as this may compromise nucleic acid quality.

4. For your safety and health, wear a lab coat and disposable gloves when handling this product.

5. Several buffers contain guanidinium salts. Do NOT mix with oxidizing disinfectants such as sodium hypochlorite; toxic gas may form. Dispose of all kit‑related waste in accordance with local institutional regulations for biohazardous waste.

6. This product is for research use only.

Storage

Part I components should be stored at -25 to -15°C for 18 months.

Part II components should be stored at 2 to 8°C for 18 months.

Part III components should be stored at room temperature for 18 months.

FAQ

Citations

Documents

Manual
18613-Manual-Ver.EN20261026.pdf

Applications

Perguntas frequentes

Legal e isenções de responsabilidade

O produto é apenas para fins de pesquisa e não se destina ao uso terapêutico ou diagnóstico em humanos ou animais. Os produtos e o conteúdo são protegidos por patentes, marcas registradas e direitos autorais de propriedade da Yeasen Biotechnology. Os símbolos de marca registrada indicam o país de origem, não necessariamente o registro em todas as regiões.

Certos aplicativos podem exigir direitos adicionais de propriedade intelectual de terceiros.

Yeasen se dedica à ciência ética, acreditando que nossa pesquisa deve abordar questões críticas, garantindo ao mesmo tempo padrões éticos e de segurança.