Tumor Necrosis Factor-alpha (TNF-α) is the most well-known member of the TNF family and can induce cell death. As a pleiotropic pro-inflammatory cytokine, TNF-α is secreted by various cell types, including adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. Belonging to the TNF ligand family, it signals through two receptors, TNFR1 and TNFR2. TNF-α exhibits cytotoxicity against a variety of tumor cells and serves as a crucial mediator in the immune response against bacterial infections. Additionally, TNF-α plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Possessing a broad spectrum of biological activities, TNF-α not only plays a central role in inflammation but also regulates apoptosis, cell proliferation, and differentiation.

Recombinant Human TNF-α is a 17.5 kDa recombinant protein containing 157 amino acid residues. Expressed in E. coli, this product is supplied as a lyophilized powder and is characterized by high activity, high purity, low endotoxin levels, and high stability.

Application

T Cell Expansion & Maintenance: Promotes T cell proliferation via NF-κB signaling and prevents functional exhaustion. Enhances transduction efficiency and supports healthy expansion in TCR-T therapies.

Dendritic Cell Maturation: Drives the differentiation of monocytes into mature dendritic cells (mDCs).

Immune Cell Activation: Enhances the anti-tumor cytotoxicity of CIK cells, NK cells, and macrophages during ex vivo expansion.

Features

High Purity: >95% purity to avoid immune responses caused by residual Host Cell Proteins (HCP) or DNA.

Ultra-Low Endotoxin: <10 EU/mg to minimize the risk of fever or inflammation.

Batch-to-Batch Consistency: Ensures stability and reproducibility in cell culture applications.

Regulatory Compliance: Meets regulatory requirements for cell therapy products and supports Investigational New Drug (IND) applications.

Animal-Origin Free (AOF): Free from animal viruses, pathogens, and exogenous factor contamination, ensuring maximum safety.

Components

Specifications

Storage

This product should be stored at -25~-15℃ for 3 years. After reconstitution, store at -85 to -65°C. Valid for 3 months.

Figure

SDS-PAGE

Figure 1. Human TNF-α GMP Protein on SDS-PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

Figure 2. The purity of Human TNF-α GMP Protein is greater than 95% as determined by SEC-HPLC.

Cell Based Assay

Figure 3. The ED50 as determined by a cytotoxicity assay using murine L929 cells is 10-50 pg/mL.

Documents:

Safety Data Sheet

90637_MSDS_HB260716

Manuals

90637_Manual_HB20260716