In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
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CLEALSCR ™ T7 ARN polymérase (faible ARNdb, 250 U / μl) _ 10628ES
Prix de venteDepuis $180.00
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ARN polymérase Cleascrip ™ T7 (grade GMP, faible ARNdb, 250 U / μl) _ 10629ES
Prix de venteDepuis $198.00
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Inhibiteur de la RNase murin GMP de qualité (40 U / μl) _ 10621ES
Prix de venteDepuis $195.00
Sauvegarder $40.00
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RNase R (grade GMP, 20 U / μl) _ 14615ES
Prix de venteDepuis $165.00
Prix régulier$205.00
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Enzyme de coiffage de la vaccina d'ARNm GMP de qualité (10 U / μl) _ 10614E
Prix de venteDepuis $365.00
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ARNm Cap 2'-O-O-méthyltransférase GMP-Grade (50 U / μL) _ 10612ES
Prix de venteDepuis $165.00
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Enzyme de plafonnement de l'ARNm Vaccinia (10 U / μl) _ 10615ES
Prix de venteDepuis $365.00
Sauvegarder $55.00
