Description
Flt-3 Ligand (Fms-related tyrosine kinase 3 ligand, FLT3L) is a key cytokine supporting the proliferation, survival, and maintenance of early hematopoietic progenitor cells and dendritic cells. In HSC expansion systems, FLT3L promotes the self-renewal and output of multipotent progenitor cells. In iPSC-to-HSC differentiation protocols, it acts during the early hematopoietic specification stage, supporting the emergence of hemogenic endothelial cells and the subsequent formation of hematopoietic progenitor cells.
Product Properties
|
Item |
Specification |
|
Synonyms |
FL, FLG3L, Flt3L, SL Cytokine |
|
Species |
Human |
|
Expressed Sequence |
Thr27-Pro185 |
|
Accession |
P49771 |
|
Tag |
No Tag |
|
Expression System |
CHO |
|
Molecular Weight |
The protein has a calculated MW of 18.0 kDa. The protein migrates as 24 kDa, 27 kDa±3 kDa when calibrated against the Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU/μg protein as determined by LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with trehalose as protectant. |
|
Appearance |
Lyophilized Powder |
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Biological Activity |
The ED50 as determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells is ≤ 2.0 ng/mL. |
|
Reconstitution Method |
It is recommended to reconstitute the protein in sterile PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles whenever possible. |
Features
- High purity: >95%, preventing immune reactions caused by residual host cell protein (HCP) or DNA.
- Ultra-low endotoxin: <10 EU/mg, minimizing the risk of fever or inflammation.
- Batch-to-batch consistency: ensuring stability and reproducibility in cell culture.
- Regulatory compliance: meeting regulatory requirements for cell therapy products and supporting IND submissions.
- Animal-origin-free: free of animal viruses, pathogenic agents, and adventitious agent contamination, ensuring high safety.
Applications
SDS-PAGE
Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.
Bioactivity
Figure 2. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 2.0 ng/mL.
Storage
Store at -25 to -15°C, valid for 3 years. After reconstitution, store at -85 to -65°C, valid for 3 months.
Paiement et sécurité
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Enquête
FAQ
Le produit est destiné à des fins de recherche uniquement et n'est pas destiné à un usage thérapeutique ou diagnostique chez l'homme ou l'animal. Les produits et le contenu sont protégés par des brevets, des marques déposées et des droits d'auteur appartenant à Yeasen Biotechnology. Les symboles de marque indiquent le pays d'origine, pas nécessairement l'enregistrement dans toutes les régions.
Certaines applications peuvent nécessiter des droits de propriété intellectuelle tiers supplémentaires.
Yeasen se consacre à la science éthique, estimant que nos recherches doivent répondre à des questions cruciales tout en garantissant la sécurité et les normes éthiques.