This kit is an enhanced system based on the principle of topoisomerase-mediated rapid and efficient DNA fragment ligation. Compared with traditional T4 DNA ligase, it offers several significant advantages.

Features

Rapid Reaction: Ligation is completed in just 1–5 minutes.

High Efficiency: Nearly 100% positive cloning rate with no self-ligation; blue-white screening is unnecessary.

Simplified Workflow: From ligation to plating takes only 15–20 minutes. The protocol eliminates ice bath, heat shock, and 1-hour recovery steps.

Broad Compatibility: Efficiently ligates DNA fragments up to 5 kb.

Specifications

Components

Shipping and Storage

This product should be stored at -25~-15℃ for 12 months.

Application

TA Cloning; Gene Expression Vector Construction; Rapid Cloning of PCR Products; Sequence Verification.

Figures

1. Principle

Figure 1. Principle of TOPO Cloning

2. Workflow

Figure 2. Workflow of TOPO Cloning

3. Superior Cloning Efficiency

Figure 2. Cloning Efficiency of Kit 10907 Across Fragment Sizes and Comparison with Supplier A*

Left(A–C): Colony formation after TA cloning using Kit 10907 with inserts of 1.2 kb (A), 3 kb (B), and 5 kb (C), showing robust cloning efficiency across a broad size range.

(D–F): PCR verification of positive clones for each insert size, confirming successful insertion of 1.2 kb (D), 3 kb (E), and 5 kb (F) fragments.

Right(A-B): Cloning comparison using 3 kb fragments. Kit 10907 (A) yielded significantly more colonies than Supplier A* (B), demonstrating superior cloning efficiency.

Documents:

Safety Data Sheet

10907_MSDS_HB250713_EN.PDF

Manuals

10907_Manual_Ver.EN20230918.pdf