Organoids and matrix gel

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Ceturegel™ Matrix Gel is a versatile extracellular matrix (ECM) preparation widely used in 2D and 3D cell culture, invasion and angiogenesis assays, in vivo tumor formation, and thrombosis research. It provides a natural, biomimetic environment that supports cell growth, adhesion, and differentiation with high reproducibility.

In organoid culture, Ceturegel™ serves as an ideal scaffold that enables stem cells to self-organize into tissue-like structures, closely mimicking in vivo conditions. With high purity, low endotoxin levels, and consistent quality, Ceturegel™ ensures reliable results across a broad range of biological and biomedical studies.

Optimized Application Protocols for Ceturegel™ Matrix Gel

Application	Description	Example Cell Type / Model	Key Features
2D Cell Culture (Matrix-Coated Surface)	Coating culture plates with diluted Ceturegel™ Matrix Gel to improve cell attachment, spreading, and physiological morphology.	Epithelial cells, endothelial cells, stem cells	Promotes natural adhesion and maintains differentiation potential under physiological stiffness.
3D Cell and Organoid Culture	Embedding cells or organoid fragments within Ceturegel™ Matrix Gel to simulate native ECM conditions.	Intestinal organoids, tumor spheroids, stem cell-derived organoids	Enables self-organization, tissue polarity, and long-term culture stability.
Cell Invasion Assay	Forming a 3D matrix barrier using Ceturegel™ Matrix Gel to assess cell migration and invasion capabilities.	Tumor cells (e.g., MDA-MB-231, A549)	Reproduces ECM remodeling and invasive behavior under controlled gradients.
Angiogenesis Assay	Using Ceturegel™ Matrix Gel as a scaffold for endothelial tube formation and vascular network modeling.	HUVECs, microvascular endothelial cells	Supports rapid capillary-like tube formation and quantitative angiogenesis analysis.
In Vivo Tumor Formation (Xenograft Model)	Mixing tumor cells with chilled Ceturegel™ Matrix Gel prior to subcutaneous or orthotopic injection in mice.	Cancer cell lines, patient-derived xenografts (PDXs)	Enhances cell viability, local retention, and tumor take rate in vivo.
Thrombosis / Coagulation Model	Employing Ceturegel™ Matrix Gel as an ECM-mimicking substrate to assess platelet adhesion and clot formation.	Endothelial or platelet adhesion models	Provides physiologically relevant matrix stiffness for coagulation and thrombosis research.

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Organoids and matrix gel

Optimized Application Protocols for Ceturegel™ Matrix Gel

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