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IL-2 R beta is a member of the cytokine receptor superfamily . Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain.
Specifications
|
Cat.No. |
96268ES25 / 96268ES60 / 96268ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96268ES25 |
96268ES60 |
96268ES76 |
|
Recombinant Cynomolgus IL-2 R beta/CD122 Protein,His Tag,Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
CD122;IL-15 R beta;IL-2 R beta;IL-2R subunit beta;IL2RB;P70-75;p75;RP5-1170K4.6;IL-15 R β;IL-2 R β;IL-2R subunit β |
|
Species |
Cynomolgus |
|
Expression Sequence |
Ala27-Asp239 |
|
Accession |
Q38J85 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.001 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
SPR Data: Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.17 μM as determined in SPR assay . |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
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