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Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. IL-3 is produced by T cells, mast cells, and eosinophils, and can enhance thrombopoiesis, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate mononuclear cells suggests that IL-3 may have additional immunomodulatory roles. Many IL-3 activities depend on co-stimulation with other cytokines.
Recombinant IL-3 is a 15.0 kDa recombinant protein containing 133 amino acid residues. This product is expressed in E. coli and provided as a lyophilized powder. It features high activity, high purity, low endotoxin, and is tag-free.
Specifications
|
Cat.No. |
90212ES10 / 90212ES50 / 90212ES60 / 90212ES80 |
|
Size |
10 μg / 50 μg / 100μg / 1 mg (10×100 μg) |
Components
|
Name |
90212ES10 |
90212ES50 |
90212ES60 |
90212ES80 |
|
Recombinant Human IL-3 GMP Protein |
10 μg |
50 μg |
100 μg |
1 mg (10×100 μg) |
Performance parameters
|
Synonyms |
Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
|
Source |
E. coli |
|
Sequence |
Ala20-Phe152. |
|
Molecular Weight |
Approximately 15.08 kDa. |
|
Accession |
P08700 |
|
Tag |
No Tag |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7×106 IU/mg |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU/μg protein as determined by LAL method. |
|
Sterility |
Negative. |
|
Mycoplasma |
Negative. |
|
In Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/ug when tested by ELISA. |
|
Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
|
Physical Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Reconstitution Method
It is recommended to reconstitute Human IL-3 in sterile PBS to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles as much as possible.
Product Data
SDS- PAGE
Human IL-3 on SDS-PAGE under reduced condition. The purity is greater than 95%.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
Storage
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