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Product Properties
|
Alternative Names |
Fibroleukin, pT49, FGL2, fibrinogen-like 2, |
|
Species |
Mouse |
|
Expression Sequence |
Pro197-Pro432 |
|
Accession |
P12804 |
|
Tag |
N-His-Avi,N-Flag |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 31.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Biotinylated Mouse FGL2, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
Storage
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