Descrição
This kit contains recombinant mouse IL-2, IL-4, IL-21, and BAFF for the in vitro polarization of mouse B cells.
IL-2 is typically added as an enhancing component to existing polarization systems. Its most important applications include synergizing with IL-4 to enhance IgG1/IgE class switching, and with TGF-β to enhance IgA class switching. In the early stages of the B cell response, it promotes clonal expansion and prepares cells for class switching; in later stages, it may influence the fate decision between plasma cells and memory B cells.
IL-4 is the core instructive factor for IgG1 and IgE class switching. In T cell-dependent responses, IL-4 is the most critical cytokine for inducing B cells to switch from expressing IgM/IgD to expressing IgG1 and IgE. Meanwhile, it is a potent B cell co-stimulatory proliferation factor, acting synergistically with BCR or CD40 signaling.
IL-21 induces B cell differentiation into plasma cells and antibody secretion.
BAFF promotes B cell survival and maturation.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Mouse B lymphocyte Polarization Cytokine Set |
92655ES10 |
10 μg |
|
92655ES50 |
50 μg |
|
|
92655ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92655ES10 |
92655ES50 |
92655ES60 |
Quantity |
|
92655ES-A |
Mouse IL-2 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-B |
Mouse IL-4 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-C |
Mouse IL-21 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92655ES-D |
Mouse BAFF Protein |
10 μg |
50 μg |
100 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
90239ES |
Mouse IL-2 Protein |
HEK293 |
Liquid |
|
90241ES |
Mouse IL-4 Protein |
HEK293 |
Liquid |
|
90233ES |
Mouse IL-21 Protein |
HEK293 |
Liquid |
|
90641ES |
Mouse BAFF Protein |
HEK293 |
Lyophilized powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Recombinant Mouse BAFF Protein |
Before opening, centrifuge to bring the contents to the bottom. Reconstitute with ultrapure water to a concentration of 0.1-1.0 mg/mL. Further dilution can be performed based on subsequent experiments. |
Performance Parameters
|
Species |
Mouse |
|
Tag |
No Tag |
|
Expression System |
HEK293 |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU per µg by the LAL method. |
|
Formulation |
PBS buffer. |
|
Appearance |
Liquid |
Storage
Store at -85 ~ -65°C. Valid for 1 year from the date of receipt.
After reconstitution, store at -85 ~ -65°C under sterile conditions for 3 months.
Product Features
High activity, high purity, high stability, low endotoxin.
Documents
Manual
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