ATP is the main energy molecule in cells and closely reflects metabolic activity. Since ATP levels are proportional to the number of viable cells, measuring ATP provides a reliable way to assess cell viability.

This kit uses Firefly luciferase, which catalyzes the reaction of luciferin with ATP and O₂ in the presence of Mg²⁺, producing light. The luminescence intensity is directly proportional to ATP concentration, and therefore to the number of viable cells.

The assay is highly sensitive, stable, and easy to use. In a 96-well format, it shows a strong linear response from 5 to 100,000 cells per well (the upper limit may vary by cell type). The procedure is fast—results are obtained in about 10 minutes—with no need for washing or medium replacement. The reagent has a long half-life and excellent stability. Compared with Calcein-AM and CCK-8 assays, the ATP-based method is simpler, faster, and more efficient.

Features

• Wide Linear Range: Shows good linearity from 100 to 20,000 cells per well in a 96-well plate, though the upper detection limit may vary slightly between cell types.
• Easy to Use: Simple protocol with ready-to-use reagents. No cell washing or medium removal required. Fast detection—results in about 10 minutes with stable readings.
• Excellent Stability: Reagents have a half-life of up to 3 hours. They remain over 90% active after 30 days at room temperature or 60 days at 2–8 °C.

Components

Shipping and Storage

This product should be stored at -25~-15℃ for 1 year.

Application

• Cell proliferation and cytotoxicity evaluation
• High-throughput drug screening
• Apoptosis and cell death detection
• Metabolic activity analysis
• Toxicology and safety assessment

Figures

1. Good linearity

 Figure 1. Excellent Linearity of ATP-Based Luminescent Assay within 100,000 Cells

The ATP Luminescent Cell Viability Assay Kit shows a strong linear correlation between luminescence intensity and cell number, maintaining good linearity up to 1 × 10⁵ cells per well.

2. Signal Stability Analysis

 Figure 2. Signal Stability Analysis within 300 Minutes

3. Strong Stability

 Figure 3. Stability Test

40211ES remains stable after up to 10 freeze-thaw cycles. Stability at 4 °C is maintained for up to 2 months, and at room temperature for up to 1 month-—significantly outperforming company P and V.

Documents:

Safety Data Sheet

40211_MSDS_HB250821_EN.PDF

Manuals

40211_Manual_Ver.EN20250820.pdf

Related Blog

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