Descrição
The HieffTM Blood DNA Kit is designed for the extraction of DNA from blood samples of mammals, poultry, birds, amphibians, and other species.
The spin columns utilize a unique silica-based matrix material proprietary to our company. When combined with our specialized lysis buffer formulation, this system enables the maximum recovery of high-purity DNA.
The extracted DNA exhibits high purity and consistent quality, making it suitable for various downstream applications such as PCR, molecular cloning, and restriction enzyme digestion.
Features
Broad compatibility: Works with anticoagulated blood, clots, and more.
High-purity gDNA: Free of protein/RNA; ready for PCR, digestion, and library prep.
Fast: <1 hour hands-on workflow.
Safe: No phenol or chloroform needed.
Components
|
Components No. |
Name |
18730ES08 (5 T) |
18730ES50 (50 T) |
18730ES70 (200 T) |
|
|
PartI |
18730-A |
Proteinase K(20 mg/ mL) |
100 µL |
1 mL |
1 mL×4 |
|
PartII |
18730-B |
HieffTM DNA Column B2 |
5 |
50 |
200 |
|
18730-C |
2 mL Collection Tube B2 |
5 |
50 |
200 |
|
|
18730-D |
AC Buffer B2 |
500 µL |
5 mL |
20 mL |
|
|
18730-E |
DB Buffer B2 |
1 mL |
10 mL |
40 mL |
|
|
18730-F |
Binding Buffer (BD Buffer B2) |
1.5 mL |
15 mL |
60 mL |
|
|
18730-G |
Protein Remove Buffer(PL BufferB2) |
2.5 mL |
25 mL |
100 mL |
|
|
18730-H |
Wash Buffer* |
1.5 mL |
13 mL |
50 mL |
|
|
18730-I |
Elution Buffer |
1 mL |
10 mL |
20 mL |
|
[Note]: Before the first use, add 4 volumes of ethanol to the Wash Buffer W* (18730-H), mix thoroughly, and label appropriately
Storage
Store Part I components at 2~8 °C, protected from light; store Part II components at room temperature. Valid for 2 years.
Figure
Higher DNA Yield
Figure 1. High RNA Integrity and Broad Compatibility Across Diverse Cultured Cell Lines Using Kit 19231.
Genomic DNA was extracted from 100 µL of mouse anticoagulated whole blood using Yeasen Cat#18730 and comparable kits from Supplier T*, O*, and G*, with elution in 200 µL nuclease-free H2O. Five microliters of each eluate were loaded on an agarose gel. Results show that Cat#18730 yields higher gDNA compared to other brands.
Documents:
Safety Data Sheet
Manuals
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