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T4 DNA Polymerase catalyzes DNA synthesis in the 5'-3' direction in the presence of a template and primer. T4 DNA Polymerase has 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity. Compared to T4 DNA Polymerase (Cat#12901ES), UCF.ME™ T4 DNA Polymerase has lower host residue, making it suitable for applications that require stricter background control, such as pathogen detection.
Applications
Smoothing of DNA 5' or 3' overhanging ends
Synthesis of labeled DNA probes via displacement reaction
Synthesis of the second strand during site-directed mutagenesis
Cloning of PCR products without reliance on ligation reactions
Specifications
|
Concentration |
3 U/μL |
|
Unit definition |
One unit of activity is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37°C |
Components
|
Components No. |
Name |
14457ES64 |
14457ES77 |
|
14457-A |
UCF.ME™ T4 DNA Polymerase (3 U/μL) |
50 μL |
250 μL |
|
14457-B |
10 × Blue Buffer |
100 μL |
250 μL |
Figures
Fig 1. Ultra-low host residue: 30 U < 3 copies
Note: Detection of E. coli genomic residue in three batches of enzyme using qPCR method, with results showing < 3 copies per 30 U
Storage
FAQ
Citations
Applications
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