High toxicity, low efficiency, and poor compatibility have long been the three major challenges in cell transfection.
Traditional transfey. For primary or sensitive cells, they often require tedious optimization of experimental conditionction reagents, such as lipid-based reagents, offer good efficiency but exhibit high toxicits. PEI-based transfection reagents, while low in toxicity, suffer from poor compatibility; apart from 293 cells, their transfection efficiency in other cell types tends to be weak.
Based on in-depth biological and materials science research, Yeasen has newly developed a next-generation polymeric transfection reagent——Hieff TransTM Booster DNA&RNA Transfection Reagent. Its unique mechanism enhances transfection efficiency at every step, delivering higher success rates for your transfection experiments!
1. Mechanism for Enhancing Transfection Efficiency
Figure 1. Mechanism Comparison between Booster and Lipo Transfection Reagent
1) High Loading Capacity
→ Dual mechanism: charge interaction + polymer chain entanglement;
Higher loading capacity than traditional charge-interaction methods.
2) Efficient Cellular Uptake
→ Membrane translocation;
Faster than conventional endocytosis pathways.
3) Non-Damaging Release
→ Rapid polymer degradation by intracellular enzymes/reducing GSH;
Zero-damage release of nucleic acids.
4) Enhanced Safety
→ Rapid polymer degradation property;
Lower toxicity than traditional lipid-based transfection reagents.
2. Product Features
- Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.
- Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).
- Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells.
- Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.
- One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.
3. Product Performance
1) High-Efficiency Primary Cell BMDM Transfection
Figure 2. Plasmid DNA transfection in primary bone marrow-derived macrophages (BMDMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
2) High-Efficiency DNA Transfection
Figure 3. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster.
3) High-Efficiency mRNA Transfection
Figure 4. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster.
4) High-Efficiency siRNA Transfection
Figure 5. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster.
4. Customer Feedback
1) High-Efficiency Primary Dermal Fibroblasts Transfection
Figure 6. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
2) High-Efficiency Porcine Alveolar Macrophages Transfection
Figure 7. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
3) High-Efficiency Primary Mouse Hepatocytes Transfection
Figure 8. Plasmid DNA transfection in Primary mouse hepatocytes using Booster DNA&RNA Transfection Reagent versus T brand L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
4) High-Efficiency Primary mouse neural stem cells Transfection
Figure 9. mRNA transfection in Primary mouse neural stem cells using Booster DNA&RNA Transfection Reagent versus S brand S*8008. The result demonstrates superior primary cell transfection efficiency of Booster.
5) DNA Plasmid Transfection: High Expression, Broad Compatibility
Figure 10. DNA transfection using Booster DNA&RNA Transfection Reagent versus T brand L*3000. The result demonstrates superior transfection efficiency of Booster.
6) Sf9 insect cells
Figure 11. DNA transfection using Booster DNA&RNA Transfection Reagent versus T brand L*2000. The result demonstrates superior transfection efficiency of Booster.
5. Ordering Information
|
Application |
Name |
Catalog No. |
Size |
|
Works with DNA, siRNA, miRNA, mRNA, and ASO, etc. Proven success in primary cells. |
40801 |
100 μL/1.5 mL |
Try it for free!
Get a free sample of Hieff Trans™ Booster and validate its performance in your own lab.
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Related Blog:
Protocol for Transfection of mRNA into Cells
Protocol for Transfection of siRNA into Cells
Protocol for Transfection of Primary Bone Marrow-Derived Macrophages (BMDMs)