Validated high-efficiency transfection protocols applicable to a wide range of cell types, designed to help you achieve optimal transfection results on the first attempt with minimal optimization. We provide specialized experimental protocols for common tissue-derived cells, covering cell growth, culture conditions, passaging procedures, and key steps for efficient transfection. These optimized protocols include detailed step-by-step instructions to achieve high transfection efficiency under low cytotoxicity conditions. Validated by numerous universities and research institutions both domestically and internationally, these solutions are highly practical and can help accelerate your research progress.
Optimized Transfection Protocols for Tissue Cell
|
Cell Line |
Species / Cell Type |
Transfection Type |
|
RAW264.7 |
DNA |
|
|
RAW264.7 |
siRNA |
|
|
Sf9 |
DNA |
|
|
BmN |
DNA |
|
|
AC16 |
DNA |
|
|
MC3T3-E1 |
DNA |
|
|
MC3T3-E1 |
siRNA |
|
|
HEK293 |
DNA |
|
|
HEK293 |
mRNA |
|
|
NIH-3T3 |
DNA |
|
|
MACT |
DNA |
|
|
HaCaT |
DNA |
|
|
DF-1 |
DNA |
|
|
Mandarin fish (Siniperca chuatsi) cells |
DNA + mRNA co-transfectionA |
