Validated high-efficiency transfection protocols applicable to a wide range of cell types, designed to help you achieve optimal transfection results on the first attempt with minimal optimization. We provide specialized experimental protocols for common tissue-derived cells, covering cell growth, culture conditions, passaging procedures, and key steps for efficient transfection. These optimized protocols include detailed step-by-step instructions to achieve high transfection efficiency under low cytotoxicity conditions. Validated by numerous universities and research institutions both domestically and internationally, these solutions are highly practical and can help accelerate your research progress.

Optimized Transfection Protocols for Tissue Cell

Cell Line

Species / Cell Type

Transfection Type

RAW264.7

Mouse monocyte–macrophage cells

DNA

RAW264.7

Mouse monocyte–macrophage cells

siRNA

Sf9

Insect ovarian cells

DNA

BmN

Silkworm (Bombyx mori) ovarian cells

DNA

AC16

Human cardiomyocyte cells

DNA

MC3T3-E1

Mouse embryonic pre-osteoblast cells

DNA

MC3T3-E1

Mouse embryonic pre-osteoblast cells

siRNA

HEK293

Human embryonic kidney cells

DNA

HEK293

Human embryonic kidney cells

mRNA

NIH-3T3

Mouse embryonic fibroblast cells

DNA

MACT

Bovine mammary epithelial cells

DNA

HaCaT

Human keratinocyte cells

DNA

DF-1

Chicken embryonic fibroblast cells

DNA

Mandarin fish (Siniperca chuatsi) cells

Mandarin fish (Siniperca chuatsi) cells

DNA + mRNA co-transfectionA

Inquiry