Transfection Solutions for Primary Cells

Validated, high-efficiency transfection protocols for a wide range of cell types. Our solutions help you achieve optimal results with minimal optimization—often right from the first attempt. We provide cell-specific protocols for both primary cells and common cell lines, covering cell growth, culture conditions, passaging, and key steps for efficient transfection. These optimized protocols include detailed step-by-step instructions to achieve high transfection efficiency with low cytotoxicity. Verified by numerous academic and research institutions worldwide, our solutions are designed to meet real-world experimental needs and help you reach your research goals faster.

Find cell line-specific protocols for primary cell lines in the table below.

Cell Name	Cell name abbreviation	Nucleic Acid Type
Primary mouse skin fibroblasts	MSFs	DNA
Primary human skin fibroblasts	HSFs	DNA
Primary mouse hepatocytes	PMHs	DNA
Primary mouse neural stem cells	mNSCs	mRNA
Primary human pulmonary artery endothelial cells	HPAECs	DNA
Primary bone marrow–derived macrophages	BMDMs	siRNA
Primary chicken embryo fibroblasts	CEFs	DNA
Primary porcine skeletal muscle satellite cells	PSCs(or PMSCs)	DNA
Primary glioblastoma cells	GBMs	SiRNA
Primary porcine alveolar macrophages	PAMs	DNA
Human Umbilical Vein Endothelial Cells	HUVEC	DNA
Primary Cardiac Fibroblasts from Neonatal Mice	PMCF	siRNA

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