D -Luciferin, kaliumsalt -40902es
D -Luciferin, kaliumsalt -40902es
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D -Luciferin, kaliumsalt -40902esD -Luciferin, kaliumsalt -40902es
D -Luciferin, kaliumsalt -40902es
D-luciferin er et almindeligt substrat for Luciferase og er meget udbredt inden for bioteknologi, især in vivo billedteknologi. Virkningsmekanismen er, at luciferin (substrat) kan oxideres til at udsende lys i nærvær af ATP og luciferase. Når fluorescein er i overskud, er kvanteantallet af produceret lys lig med Luciferase-koncentrationer var positivt korreleret (se figur nedenfor). Plasmider, der bærer luciferase-kodende gen (Luc), blev transficeret ind i celler og indført i undersøgelsesdyr, såsom rotter og mus. D-luciferin er også almindeligt anvendt i in vitro undersøgelser, herunder luciferase- og ATP-niveauanalyse; Reporter genanalyse; High-throughput sekventering...

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D -Luciferin, kaliumsalt -40902es

YeasenSKU: 40902ES01
Størrelse: 100 mg
Pris:
$100.00
Lager:
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D-luciferin er et almindeligt substrat for Luciferase og er meget udbredt inden for bioteknologi, især in vivo billedteknologi. Virkningsmekanismen er, at luciferin (substrat) kan oxideres til at udsende lys i nærvær af ATP og luciferase. Når fluorescein er i overskud, er kvanteantallet af produceret lys lig med Luciferase-koncentrationer var positivt korreleret (se figur nedenfor). Plasmider, der bærer luciferase-kodende gen (Luc), blev transficeret ind i celler og indført i undersøgelsesdyr, såsom rotter og mus.

D-luciferin er også almindeligt anvendt i in vitro undersøgelser, herunder luciferase- og ATP-niveauanalyse; Reporter genanalyse; High-throughput sekventering og forskellige kontamineringstests. Der er tre produktformer: D-luciferin (fri syre), D-luciferinsalt (natriumsalt og kaliumsalt). Den største forskel ligger i opløseligheden: vandopløseligheden af ​​førstnævnte og opløseligheden af ​​buffersystemet. De er svagt opløselige undtagen i svage baser såsom lave koncentrationer af NaOH og KOH opløsninger. Opløselig i methanol og DMSO; Sidstnævnte kan let opløses i vand eller buffer, let at bruge.

Funktioner

  • Ingen stråling, næsten uskadelig for levende organismer.
  • Bioluminescens, ingen excitationslyskilde.
  • Så følsom, du kan opdage det i et par hundrede celler.
  • God penetration, 3-4 cm vævsdybde kan stadig detekteres.
  • Højt signal-til-støj-forhold, stærkt fluorescenssignal og god anti-interferens.

Ansøgninger

  • I tumorgenese-eksperimentet i nøgne mus blev tumorvæksten observeret uden invasion i realtid uden tumorstrippingsmåling.
  • For at teste virkningen af ​​indgivelsen på tumorvækst eller metastase kan fluoresceinsubstratet elimineres fuldstændigt inden for 3 timer uden indblanding i lægemiddelforsøget.
  • Lokaliseringen og fordelingen af ​​fremmede celler i dyr blev påvist.
  • Målgenet eller promotoren af ​​målgenet fusioneres til luciferasegenet for at detektere ændringer i genekspression under lægemiddelbehandling eller sygdomsprogression.
  • Overvågning af stamcelletransplantation, overlevelse og spredning; Spor distributionen og migrationen af ​​stamceller in vivo.

Specifikationer

engelsk synonym (S)-4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolcarboxylsyrekaliumsalt; D-Luciferin ildflue, kaliumsalt
CAS NR. 115144-35-9
Formel C11H7N2O3S2K
Molekylvægt 318,42 g/mol
Udseende Lysegult pulver
Opløselighed Opløseligt i vand (60 mg/ml)

Komponenter

Komponenter nr. Navn 40902ES01 40902ES02 40902ES03 40902ES08
40902 D-Luciferin, Kaliumsalt 100 mg 500 mg 1g 5g


Figurer

Citeret fra "A Novel Allosteric Inhibitor of Phosphoglycerat Mutase 1 undertrykker vækst og metastase af ikke-småcellet lungekræft, Cell Metab.2019;30(6):1107-1119

Forsendelse og opbevaring

Produktet sendes med tøris og kan opbevares ved -15℃ ~ -25℃ for 1 år.

Dokumenter:
Citater og referencer:

[1] Gu Y, Wang Y, He L, et al. Cirkulært RNA circIPO11 driver selvfornyelse af leverkræft-initierende celler via Hedgehog-signalering. Mol Cancer. 2021;20(1):132. Udgivet 14. oktober 2021. doi:10.1186/s12943-021-01435-2(IF:27.401)

[2] Huang K, Liang Q, Zhou Y, et al. En ny allosterisk hæmmer af phosphoglycerat Mutase 1 undertrykker vækst og metastase af ikke-småcellet lungekræft [publiceret korrektion vises i Cell Metab. 2021 Jan 5;33(1):223]. Celle Metab. 2019;30(6):1107-1119.e8. doi:10.1016/j.cmet.2019.09.014(IF:22.415)

[3] Dong X, Cheng R, Zhu S, et al. En heterojunction-struktureret WO<sub>2.9</sub>-WSe<sub>2</sub> nanoradiosensibilisator øger lokal tumorablation og kontrolpunktblokade-immunterapi ved lav strålingsdosis. ACS Nano. 2020;14(5):5400-5416. doi:10.1021/acsnano.9b08962(IF:14.588)

[4] Zheng DW, Gao F, Cheng Q, et al. Et vaccinebaseret nanosystem til at starte medfødt immunitet og forbedre tumorimmunterapi. Nat Commun. 2020;11(1):1985. Udgivet 24. april 2020. doi:10.1038/s41467-020-15927-0(IF:12.121)

[5] Hu J, Su H, Cao H, et al. AUXIN RESPONSE FACTOR7 integrerer gibberellin og auxin signalering via interaktioner mellem DELLA og AUX/IAA proteiner for at regulere kambial aktivitet i poppel. Plantecelle. 2022;34(7):2688-2707. doi:10.1093/plcell/koac107(IF:11.277)

[6] Qi P, Huang M, Hu X, et al. En Ralstonia solanacearum-effektor retter sig mod TGA-transkriptionsfaktorer for at undergrave salicylsyresignalering. Plantecelle. 2022;34(5):1666-1683. doi:10.1093/plcell/koac015(IF:11.277)

[7] Huang X, Qiu M, Wang T, et al. Bærerfri multifunktionel nanomedicin til intraperitoneal dissemineret ovariecancerterapi. J Nanobioteknologi. 2022;20(1):93. Udgivet 22. februar 2022. doi:10.1186/s12951-022-01300-4(IF:10.435)

[8] Tang Y, Lin S, Yin S, et al. In situ gasskumning baseret på magnesiumpartikelnedbrydning: En ny tilgang til fremstilling af injicerbare makroporøse hydrogeler. Biomaterialer. 2020;232:119727. doi:10.1016/j.biomaterials.2019.119727(IF:10.273)

[9] Zhang X, Zheng S, Hu C, et al. Kræftassocieret fibroblast-induceret lncRNA UPK1A-AS1 giver platinresistens i bugspytkirtelkræft via effektiv dobbeltstrengsbrudreparation. Onkogen. 2022;41(16):2372-2389. doi:10.1038/s41388-022-02253-6(IF:9.867)

[10] Qiao K, Liu Y, Xu Z, et al. RNA m6A-methylering fremmer dannelsen af ​​vaskulogen mimik i hepatocellulært karcinom via Hippo-vejen. Angiogenese. 2021;24(1):83-96. doi:10.1007/s10456-020-09744-8(IF:9.780)

[11] Wu Q, Kuang K, Lyu M, et al. Allosterisk deaktivering af PIF'er og EIN3 af mikroproteiner i lyskontrol af planteudvikling. Proc Natl Acad Sci US A. 2020;117(31):18858-18868. doi:10.1073/pnas.2002313117(IF:9.412)

[12] Meng DF, Sun R, Liu GY, et al. S100A14 undertrykker metastase af nasopharyngeal carcinom ved hæmning af NF-kB-signalering gennem nedbrydning af IRAK1. Onkogen. 2020;39(30):5307-5322.doi:10.1038/s41388-020-1363-8(IF:7.971)

[13] Gao Y, Wang J, Han H, et al. Et nanopartikelholdigt polycaprolactonimplantat til bekæmpelse af tilbagefald af brystkræft efter resektion. Nanoskala. 2021;13(34):14417-14425. Udgivet 2. september 2021. doi:10.1039/d1nr04125h(IF:7.790)

[14] Jiang W, Li T, Guo J, et al. Bispecifikke c-Met/PD-L1 CAR-T-celler har forbedret terapeutisk effekt på hepatocellulært karcinom. Front Oncol. 2021;11:546586. Udgivet 2021 10. marts. doi:10.3389/fonc.2021.546586(IF:6.244)

[15] Zhou Y, Zhou C, Zou Y, et al. Multi pH-følsomme polymer-lægemiddelkonjugat blandede miceller til effektiv samtidig levering af doxorubicin og curcumin for synergistisk at undertrykke tumormetastaser. Biomater Sci. 2020;8(18):5029-5046. doi:10.1039/d0bm00840k(IF:6.183)

[16] Zhao X, Liu X, Zhang P, et al. Injicerbar peptidhydrogel som intraperitonealt triptoliddepot til behandling af ortotopisk hepatocellulært karcinom. Acta Pharm Sin B. 2019;9(5):1050-1060. doi:10.1016/j.apsb.2019.06.001(IF:5.808)

[17] Li M, Wang J, Yu Y, et al. Karakterisering af mesenkymale stamceller afledt af bisfosfonat-relateret osteonekrose af kæbepatienters tandkød. Stem Cell Rev Rep. 2022;18(1):378-394. doi:10.1007/s12015-021-10241-8(IF:5.739)

[18] Zhang X, Li Y, Ji J, et al. Gadd45g initierer embryonal stamcelledifferentiering og hæmmer brystcellecarcinogenese. Celledød Discover. 2021;7(1):271. Udgivet 2. oktober 2021. doi:10.1038/s41420-021-00667-x(IF:5.241)

[19] Wu D, Lv J, Zhao R, et al. PSCA er et mål for kimæriske antigenreceptor-T-celler i mavekræft. Biomark Res. 2020;8:3. Udgivet 2020 28. januar. doi:10.1186/s40364-020-0183-x(IF:4.866)

[20] Qiao K, Chen C, Liu H, Qin Y, Liu H. Pinin inducerer epitel-til-mesenkymal overgang i hepatocellulært karcinom ved at regulere m6A-modifikation. J Oncol. 2021;2021:7529164. Udgivet 7. december 2021. doi:10.1155/2021/7529164(IF:4.375)

[21] Li K, Liu T, Chen J, Ni H, Li W. Survivin i brystkræft-afledte exosomer aktiverer fibroblaster ved at opregulere SOD1, hvis feedback fremmer cancerproliferation og metastasering. J Biol Chem. 2020;295(40):13737-13752. doi:10.1074/jbc.RA120.013805(IF:4.238)

[22] Kong Y, Feng Z, Chen A, et al. Den naturlige flavonoid galangin fremkalder apoptose, pyroptose og autofagi i glioblastom. Front Oncol. 2019;9:942. Udgivet 2019 27. september doi:10.3389/fonc.2019.00942(IF:4.137)

[23] Kong Y, Feng Z, Chen A, et al. Den naturlige flavonoid galangin fremkalder apoptose, pyroptose og autofagi i glioblastom. Front Oncol. 2019;9:942. Udgivet 2019 27. september doi:10.3389/fonc.2019.00942(IF:4.137)

[24] Hu J, Wang Y, Yuan Y. Inhibitorer af APE1 redoxfunktion hæmmer effektivt γ-herpesvirus replikation in vitro og in vivo. Antiviral Res. 2021;185:104985. doi:10.1016/j.antiviral.2020.104985(IF:4.101)

[25] Li Q, Wei D, Feng F, et al. α2,6-bundet sialinsyre tjener som en højaffinitetsreceptor for cancer onkolytisk viroterapi med Newcastle disease-virus. J Cancer Res Clin Oncol. 2017;143(11):2171-2181. doi:10.1007/s00432-017-2470-y(IF:3.503)

[26] Han B, Jiang P, Liu W, et al. Daucosterollinoleats rolle på brystkræft: undersøgelser af apoptose og metastase. J Agric Food Chem. 2018;66(24):6031-6041. doi:10.1021/acs.jafc.8b01387(IF:3.412)

[27] Hu H, Zhang Z, Wang R, et al. BGC823-cellelinje med det stabile udtryk af iRFP720 bevarer sine primære egenskaber med lovende fluorescensbilleddannelsesevne. DNA Cell Biol. 2020;39(5):900-908. doi:10.1089/dna.2019.5057(IF:3.314)

[28] Ni XR, Zhao YY, Cai HP, et al.Transferrinreceptor 1 målrettet optisk billeddannelse til identifikation af gliommargin i musemodeller. J Neuroncol. 2020;148(2):245-258. doi:10.1007/s11060-020-03527-3(IF:3.267)

[29] Feng H, Tang J, Zhang P, Miao Y, Wu T, Cheng Z. Anti-adipogene 18,19-seco-ursane stereoisomerer og olean-type saponiner fra Ilex cornuta blade. Fytokemi. 2020;175:112363. doi:10.1016/j.phytochem.2020.112363(IF:3.044)

[30] Zhao Y, Li T, Tian S, et al. Effektiv inhibering af MYC-amplificeret gruppe 3 medulloblastom gennem målretning mod EIF4A1. Cancer Manag Res. 2020;12:12473-12485. Udgivet 3. december 2020. doi:10.2147/CMAR.S278844(IF:2.886)

Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.

FAQ

There is no difference. The three are merely variations in naming by different companies, and they are all compounds (S)-2-(6-Hydroxy-2-benzothiazolyl)-2 -thiazoline-4-carboxylic acid
Besides being used for in vivo imaging, fluorescent dye products are also employed in other applications involving luciferase, such as in vitro reporter gene assays, microbial/viral monitoring, pyrosequencing, etc.
Sodium fluorescein: It can be used for cell permeability testing. The permeability of the pigment can be determined by measuring the absorbance value at OD490. D-fluorescein sodium salt: Used for in vivo imaging and reporter gene systems. It is detected through a cold luminescence module and does not require excitation light stimulation.
The main differences among the three are as follows:
1) Solubility: The salt form is easily soluble in water. The solubility of potassium salt is 60mg/ml, and that of sodium salt is 100mg/ml. Free acid is not easily soluble in water. It can be dissolved by a weak alkaline solution of sodium bicarbonate, and its solubility in methanol is 10mg/ml, and in DMSO is 50mg/ml.
2) Toxicity: The salt form is more convenient to use during the process, especially in in vivo imaging experiments, as it can dissolve in water and the reaction toxicity will be smaller (Fluorescein is a low-molecular-weight organic compound composed of phenylthiazole and thiazole carboxyl group, with low toxicity).
3) Usage effect: There is no significant difference. In in vivo experimental studies, the usage rate of potassium salt is higher.
It has an impact. The purity is above 99%, with a good level of 99%. For 99% pure fluorescein, there is 1% solid impurity. If this 1 gram of impurity is dissolved in 25 ml of buffer solution (this dilution ratio is the standard dilution method for in vivo imaging experiments), the concentration of the impurity at this time is 0.4 g/L. Assuming the molecular weight of the impurity is 1000 g/mol, then the molar concentration of the impurity is 400 μM. This concentration may inhibit the action of certain enzymes in the cells and may reduce the experimental effect or cause harm to the animals.
The powder should be stored in a dark place at -20 or -70 degrees Celsius. Its validity period is at least one year.
D-fluorescein (D-Luciferin) is a commonly used substrate for luciferase. Its mechanism of action is that under the action of ATP and luciferase, the fluorescent substance (the substrate) can be oxidized and emit light. When there is an excess of fluorescent substance, the number of photons produced is positively correlated with the concentration of luciferase.
2. Native coelenterazine is an action substrate for various luciferases such as sea kidney luciferase (Rluc) and Gaussia luciferase (Gluc).
Recommended instruments:
1. Instruments with a biochemiluminescence detection module. The light produced by luciferin can be detected by a photometer or a scintillation counter. Common in vivo imaging instruments: such as the IVIS® Lumina small animal in vivo imaging system, the In-Vivo Xtreme multi-mode small animal in vivo imaging instrument from Bruker, Germany.
2. Multi-functional microplate readers: It is necessary to confirm with the instrument manufacturer whether it is a specific biochemiluminescence detection module (note: cannot use a fluorescence microscope).
Fluorescein is a small molecule with excellent water solubility and lipid solubility. It can easily penetrate the cell membrane and the blood-brain barrier.
Either one is fine. Intraperitoneal injection has a slower diffusion rate and the light emission lasts longer; intravenous injection has a faster diffusion rate, but the light emission lasts for a very short period of time.
Intraperitoneal injection (i.p.), with a fluorescein concentration of 150 mg/kg per kg of body weight. Taking a 20g C57 mouse as an example, the dosage for each mouse for one test: 150 mg/kg × 20g = 3mg, 100mg ÷ 3mg = 33 mice. This is for reference only. The specific dosage should be based on the reagent and without any loss.
Generally speaking, potassium d-fluorescein does not cause any toxic effects on animals.
There is no such information.
This product undergoes in vitro imaging. The ATP and magnesium ions required for this process need to be achieved through the cell culture medium. Therefore, a cell culture medium containing ATP and magnesium ions needs to be added to dilute the probe;
2. The cells themselves also contain a certain amount of ATP and magnesium, which can promote the reaction. In most cases, the ATP and magnesium contained in the cells are sufficient for the reaction of D-fluorescein salt, but the content varies depending on the type of cells. You can first try using a culture medium without additional ATP and magnesium, and conduct the experiment normally to see the results.
Chemiluminescence, no excitation wavelength required. The detection wavelength for firefly luciferin is 560 nm. The detection wavelength for sea kidney luciferin is 465 nm.
It can pass through the blood-brain barrier, the placental barrier and the blood test barrier, and also enter living cells.
The recommended concentrations are derived from the literature:
1) Injection method: It can be done through intraperitoneal injection or tail vein injection.
2) Injection volume: The scientific approach is to evaluate the injection dose based on the kinetic curve. The initial attempted injection dose is: 150mg substrate/kg of mouse body weight. Therefore, the purchase quantity can be calculated according to the above method: If there are 10 mice, 22 - 25g, then 33 - 37.5mg of substrate is required.
Approximately 3 minutes after intraperitoneal injection of fluorescein into mice, cells expressing luciferase began to emit light. The intensity reached a stable peak after 10 minutes, and this peak lasted for about 10 - 15 minutes before gradually diminishing. Detection can be conducted within 10 - 15 minutes after the injection. This is for reference only. It is recommended to conduct a preliminary experiment to establish the kinetics curve of luciferase, thereby determining the optimal time for signal detection and the signal plateau period.
To successfully conduct the in vivo imaging experiment, the following conditions are required: The target tissue or cells must express the luciferase gene; the injection of the luciferin substrate must be successful; and the thickness of the tissue at the luminescent site is also a factor. If the experiment fails, the reasons can be investigated from these aspects: whether the luciferase gene is expressed, whether the luciferin substrate was injected correctly, and whether the luminescent site is deep.

Citations

Documents

Brochure
In_vivo_imaging_Leaving_Tumor_Cells_Nowhere_to_Hide.pdf

Applications

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