In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
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Yeasen
CleaScrip ™ T7 RNA -polymerase (lavt dsRNA, 250 U/μL) _ 10628ES
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Yeasen
Cleascrip ™ T7 RNA-polymerase (GMP-klasse, lavt dsRNA, 250 U/μl) _ 10629ES
SalgsprisFra $0.00
Regelmæssig pris$198.00
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