Beskrivelse
The HieffTM 5 min Flash Cell RNA Kit is designed for total RNA extraction from cultured cells grown in 96-, 24-, 12-, or 6-well plates, with a procedure time of only 5 minutes. The spin adsorption columns utilize a unique silica-based matrix material developed by our company. Combined with our proprietary buffer system, this kit effectively removes contaminants such as cellular metabolites and proteins to the greatest extent possible. The purified RNA exhibits high purity and consistent quality, making it suitable for various downstream applications, including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning.
Features
- Ultra-Fast: RNA extraction in under 5 minutes per sample.
- High RNA Integrity: Proprietary formulation preserves RNA integrity throughout the workflow.
- Broad Compatibility: Works efficiently with challenging samples, including primary cells, protein-rich muscle tissue, lipid-rich brain tissue, and aquatic species (e.g., fish and shrimp).
- Efficient gDNA Removal: Integrated DNA-removing/RNA-binding column eliminates genomic DNA contamination in just 30 seconds—no DNase required.
Components
|
Components No. |
Name |
19231ES08 (5 T) |
19231ES50 (50 T) |
|
19231-A |
HieffTM DNA removing/RNA binding Column A2 |
10 pieces |
100 pieces |
|
19231-B |
2 mL Collection Tube C2 |
10 pieces |
100 pieces |
|
19231-C |
Lysis Buffer LB*(LB Buffer C2*) |
3 mL |
30 mL |
|
19231-D |
Protein Removal Buffer PL(PL Buffer C2) |
4 mL |
40 mL |
|
19231-E |
Wash Buffer W* (Wash Buffer C2*) |
1.3 mL |
13 mL |
|
19231-F |
RNase-free H2O |
1 mL |
5 mL |
[Note]: For 19231ES08-E Wash Buffer W*: Add 5.2 mL absolute ethanol before first use.
For 19231ES50-E Wash Buffer W*: Add 52 mL absolute ethanol before first use.
Mix well after addition and store as directed.
Storage
Store at room temperature, protected from light. Stable for 2 years. For longer-term storage, keep at 2–8℃.
Figure
High RNA Integrity
Figure 1. High RNA Integrity and Broad Compatibility Across Diverse Cultured Cell Lines Using Kit 19231.
RNA was extracted from various cultured cell types using the HieffTM Flash RNA Kit (Cat. No. 19231) and analyzed by agarose gel electrophoresis. All samples showed sharp, distinct 28S and 18S rRNA bands with an approximate intensity ratio of 2:1, indicating excellent RNA integrity and consistent performance across cell types.
Documents:
Safety Data Sheet
Manuals
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