Hifair™ I Multiplex One Step RT-qPCR Lyophilized Microsphere _ 16936ES

YeasenSKU: 16936ES16

Size: 16 T
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Beskrivelse

This product is a lyophilized micro-core (primers and probes not included) prepared from the liquid lyo-ready formulation of the Hifair™ Lyo-Ready Multiplex One Step RT-qPCR Kit (UDG plus)(Cat#16645ES). It enables multiplex quantitative PCR reactions using RNA as a template. By combining reverse transcription and quantitative PCR in a single reaction tube, this kit simplifies experimental procedures and minimizes the risk of contamination.

Utilizing advanced lyophilization technology, this product transforms liquid reagents into stable lyophilized micro-cores. This design significantly facilitates storage and transportation while enhancing the overall stability of the reagents.

Features

  • Broad Compatibility: Suitable for direct detection or freeze-dried for long-term storage
  • Strong stability: after freeze-drying, the stability is still good at 50℃ for 25 days
  • Support fast program: the fastest 40 minutes to get the result
  • Significant anti-pollution effect: UDG/dUTP anti-pollution system, easy to remove contamination.

Application

Virus detection; pathogen detection; gene expression analysis

Specifications

Cat.No.

16936ES16 / 16936ES96

Size

16 T / 96 T

Components

Components No.

Name

16936ES16

16936ES96

16936

8-Strip Lyophilized Micro-core

16 Units

96 Units

Storage

This product should be stored at -25~-15℃ for one year. 

Figures

1. Freeze-dried products form 

Figure 1. Freeze-dried product form(Eight-row freeze-dried micropheres) Figure 1. Freeze-dried product form(Eight-row freeze-dried micropheres)

2. Consistent performance

Figure 2. Consistent performance between Liquid reagent and lyophilized Microspheres

Figure 2. Consistent performance between Liquid reagent and lyophilized Microspheres

3. Stability test—Appearance stability of freeze-dried microspheres

Figure 3. Freeze-dried microspheres accelerate stability((fast procedure) Figure 3. Freeze-dried microspheres accelerate stability((fast procedure)

 

After being freeze-dried into freeze-dried microspheres using Yeasen's RNA lyophilizable reagent (Catt#16936ES),  placed under different conditions (accelerated at 50°C for 33 days), and 104, and 103 copies/mL of HPIV pseudovirus templates were amplified simultaneously. The results showed that there was no significant change in the Ct value, fluorescence value, spherical shape, etc. of the freeze-dried microspheres before and after thermal acceleration, and the freeze-dried product had good stability.

4. Mutiplex PCR Amplification of pathogens

Figure 4. Multiplex amplification of pathogens  (HPIVs, Template concentration E6/5/4/3/2/1)

Figure 4. Multiplex amplification of pathogens

(HPIVs, Template concentration E6/5/4/3/2/1) 

Documents:

Safety Data Sheet

16936_MSDS_HB20260408.PDF

Manuals

16936_Manual_HB20260407.pdf

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