2×Hieff™ Multiplex PCR Master Mix for NGS _ 13606ES
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2×Hieff™ Multiplex PCR Master Mix for NGS _ 13606ES
2×Hieff™ Multiplex PCR Master Mix for NGS _ 13606ES
The Hieff™ Multiplex PCR Master Mix for NGS is a ready-to-use 2× premix developed for multiplex PCR applications. It contains a hot-start, multiplex-optimized Taq DNA polymerase blend and a proprietary amplification buffer system. The master mix enables rapid and convenient multiplex PCR and supports efficient amplification of targets with GC content ranging from 25% to 75%. It delivers high uniformity, specificity, and sensitivity across multiple amplicons. This reagent is suitable for multiplex amplification of 30–300 amplicons of different sizes and can also be used for capture-based assays involving thousands or...

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2×Hieff™ Multiplex PCR Master Mix for NGS _ 13606ES

YeasenSKU: 13606ES03
Size: 1 mL
Pris:
$285.00
Lager:
På lager

The Hieff™ Multiplex PCR Master Mix for NGS is a ready-to-use 2× premix developed for multiplex PCR applications. It contains a hot-start, multiplex-optimized Taq DNA polymerase blend and a proprietary amplification buffer system.

The master mix enables rapid and convenient multiplex PCR and supports efficient amplification of targets with GC content ranging from 25% to 75%. It delivers high uniformity, specificity, and sensitivity across multiple amplicons.

This reagent is suitable for multiplex amplification of 30–300 amplicons of different sizes and can also be used for capture-based assays involving thousands or more amplicons. It is widely applicable in animal and plant breeding, forensic identification, genetic disease testing, food safety analysis, and related fields.

Features

Easy to operate: Ready-to-use 2× premixed solution

Broad Compatibility: Suitable for complex samples, GC-rich/poor systems(25-75%), and multiplex amplification.

Excellent performance: High uniformity, specificity, and sensitivity; supports 30–300 multiplex amplicons or enrichment of thousands of targets.

Components

Components No.

Name

13606ES03

13606ES08

13606ES50

13606ES65

13606

2 × Hieff™ Multiplex PCR Master Mix for NGS

1 mL

5 mL

50 mL

65 mL

Application

Targeted gene panels: Amplify disease-related genes (e.g., cancer, inherited disorders).

Direct blood PCR: No DNA extraction needed.

Forensic identification: Type STRs/SNPs for identity.

Agricultural breeding: Detect trait-associated markers (e.g., SNPs, indels).

Figures

1. High Amplification Efficiency

NO.

DNA

Concentration(ng/uL）

Supplier B*

(ng/uL）

Yeasen

(ng/uL）

SNP-1

34.8

4.66

41.2

SNP-2

67.8

5.5

46.8

SNP-3

46.6

8.28

44.4

SNP-4

45.8

8.54

44

SNP-5

66.4

7.44

43.2

SNP-6

69.2

6.62

44.4

SNP-7

102

10.8

38

SNP-8

70.6

9.86

44

Figure 1. Multiplex amplification test using 13606ES for 300-plex SNP genotyping. Amplicons (100–200 bp) show high specificity and uniformity across all targets.

Storage

This product should be stored at -25~-15℃ for 2 years.

FAQ

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FAQ

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