RNA Library Preparation Solutions
High Exon Coverage Uniformity RNA Library Prep Kit
Compatiable with illumina, MGI and Element sequencing platform
Key Performance Data
Less to 5 Tubes, Save 2 Hours
(A). Less to 5 Tubes
We refined the formulation and simplified the steps for first-strand synthesis, second-strand synthesis, and ligation, enabled by rigorous development and validation testing.
(B). Operation Time Reduced by 2 Hours
Enzymes and buffers are pre-mixed to reduce reagent components and simplify preparation.
Enhanced Strand-specificity for Reliable Transcript Profiling
(A). Actinomycin D–Free, Lab-Safe workflow
No toxic Actinomycin D, no light-sensitive steps, and no unpleasant odor exposure are required.
This safer workflow also delivers high strand-specific data, ensuring reliable RNA library preparation under standard lab conditions.
(B). Optimized Reverse Transcriptase for Accurate Strand Information
A novel, advanced reverse transcriptase fully suppresses intrinsic DNA polymerase activity, enhancing strand fidelity. This ensures accurate discrimination of sense and antisense transcripts and supports reliable gene expression and transcriptome analysis.
Enhanced Exon Coverage Uniformity
(A). Higher Library Yield
Optimized enzymatic performance significantly boosts library yield and conversion efficiency, providing superior library output and higher complexity in both conventional mRNA libraries and lncRNA libraries, while ensuring reliable transcriptome representation.
(B). High Exon Coverage Uniformity
Delivers even coverage across exonic regions with minimal 5′/3′ bias. Consistent performance across different transcript lengths and GC contents ensures accurate quantification and reproducible results
(C). Robust High-GC Coverage
Delivers improved sequencing depth across high-GC exonic regions. Consistent performance across genes with varying GC content ensures accurate quantification and reliable detection. RNA-seq profiling (~8,000 genes) demonstrates enhanced coverage in high-GC exons with Yeasen 12340.
Automation-Ready with Fewer Cleanups and Handling Steps
Designed with streamlined plate-format packaging, this kit is fully compatible with major commercial automation workstations. It minimizes manual cleanups and handling steps, enabling scalable high-throughput processing with consistent batch performance.
(A). Higher Library Yield
(B). Consistency Analysis
Figure . Comparison of Library Yields Between Automated and Manual Prep.
(A).Four RNA standard samples (D5, D6, F7, M8) were used (3 replicates each) with 12340 and mRNA Isolation Master Kit (Cat#12629). Libraries were prepared using the automated platform and manual workflow. Both automated and manual preps showed highly uniform library yields across all samples.
(B).Accuracy of gene expression quantification.
Fold-change analysis of 12340 against the RNA standard dataset showed high consistency (Pearson r2 > 0.94).
Application
Yeasen provides application-driven NGS workflows designed to support diverse research and clinical needs—from fundamental genomics to translational and applied sequencing studies.
Transcriptome Profiling
mRNA Sequencing
Genome-wide gene expression analysis through poly(A)+ RNA enrichment for transcript quantification and differential expression studies.
Total RNA Sequencing
Comprehensive transcriptome analysis including coding and non-coding RNA by removing rRNA.
LncRNA Sequencing
Identification and quantification of long non-coding RNAs involved in gene regulation.
Alternative Splicing Analysis
Detection and characterization of transcript isoforms and alternative splicing events.
Specialized & Advanced RNA Applications
Pathogen/Microbial Transcriptomics
Analysis of microbial gene expression and host–pathogen interactions during infection.
Small RNA Sequencing
Profiling of regulatory small RNAs such as miRNA, siRNA, and piRNA.
FFPE / Degraded RNA Sequencing
Transcriptome analysis from degraded RNA samples such as FFPE tissues.
Fusion Transcript Detection
Identification of gene fusion transcripts and chimeric RNA molecules in cancer research.
Single-Cell Transcriptomics
Gene expression analysis at single-cell resolution to reveal cellular heterogeneity.
Product Selection Guide
Specification | Conventional RNA seq Kit | Strand-specific Kit | Non-strand-specific Kit |
Cat.NO. | |||
Input RNA Range | 10 ng – 1 µg | 10 ng – 4 µg | 10 ng – 4 µg |
Workflow Time | ~4 h | ~3.5 h | ~3.5 h |
Tube Qty | 10 tubes | 5 tubes | 5 tubes |
mRNA enrichment | Compatible with mRNA Oligo dT Beads 12629ES |
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rRNA depletion | Compatible with rRNA Depletion Module 12257ES (Human/Rat/Mouse) or 12254ES (Plant) |
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Strand-specific | Strand-specific & | ✔ | × |
Automation compatibility | ✔ | ✔ | ✔ |
Customer Cases & Protocols
FAQs
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