Tanım
THP-1 cells are a human monocytic leukemia cell line and a classic in vitro model for studying macrophage function. In experiments, cytokine stimulation is a common method for inducing THP-1 cell differentiation. Cytokines such as IL-4 and IL-13 can promote the differentiation of THP-1 cells into macrophages and initiate specific signaling pathways, thereby regulating cell function. This method can simulate in vivo immunomodulatory processes and provide a convenient way to study the functions of macrophages in inflammatory responses, immunomodulation, and anti-tumor activities.
Macrophages are classified into two main types: M1-type macrophages and M2-type macrophages. THP-1 cells are usually induced to differentiate into macrophages using phorbol ester (PMA), and then, in the continued presence of PMA: lipopolysaccharide (LPS) and IFN-γ are added and cultured for 48 hours to induce M1 polarization; IL-4 and IL-13 are added and cultured for 48 hours to induce M2 polarization. Finally, the cells are resuspended in serum-free RPMI-1640 basal medium without stimulants and PMA, and can be maintained for 72 hours.
This kit is used to differentiate THP-1 cells into macrophages (M1 or M2 type). This kit contains five reagents: recombinant human IL-13, recombinant human IL-4, recombinant human IFN-γ, phorbol ester (PMA), and lipopolysaccharide (LPS). PMA is used to initially differentiate THP-1 monocytes into unpolarized M0 macrophages. IFN-γ and LPS initiate the core signals for M1 polarization. IL-4 and IL-13 act synergistically to drive cell polarization towards the M2 phenotype.
Product Information
|
Product Information. |
Cat. NO. |
Size |
|
Human THP-1 Cell Polarization Induction Kit |
92640ES08 |
5 μg |
|
92640ES50 |
50 μg |
|
|
92640ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92640ES10 |
92640ES50 |
92640ES60 |
|
92640ES-A |
Human IL-4 Protein |
5 μg |
50 μg |
100 μg |
|
92640ES-B |
Human IL-13 Protein |
10 μg |
50 μg |
100 μg |
|
92640ES-C |
Human IFN-γ Protein |
5 μg |
50 μg |
100 μg |
|
92640ES-D |
Lipopolysaccharide (LPS) |
5 mg |
5 mg |
5 mg |
|
92640ES-E |
PMA |
1 mg |
1 mg |
1 mg |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
Quantity |
|
90105ES |
Human IL-4 Protein |
E.coli |
Lyophilized Powder |
1 |
|
90112ES |
Human IL-13 Protein |
E.coli |
Lyophilized Powder |
1 |
|
91207ES |
Human IFN-γ Protein |
E.coli |
Lyophilized Powder |
1 |
|
60747ES |
Lipopolysaccharide (LPS) |
E.coli |
Lyophilized Powder |
1 |
|
50601ES |
PMA |
Plant |
Powder |
1 |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Human IL-4 Protein |
Before opening the cap, centrifuge first to bring the contents to the bottom. Reconstitute using sterile water to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human IL-13 Protein |
|
|
Human IFN-γ Protein |
|
|
Lipopolysaccharide (LPS) |
Reconstitute with water or cell culture medium to 1 mg/mL. |
|
PMA |
Dissolve in DMSO or Ethanol |
Storage
|
Component Name |
Storage Conditions |
|
Human IL-4 Protein |
Store at -25 ~ -15°C. Valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C under sterile conditions, valid for 7 days. After reconstitution, store at -85 ~ -65°C under sterile conditions, valid for 3 months. |
|
Human IL-13 Protein |
|
|
Human IFN-γ Protein |
|
|
Lipopolysaccharide (LPS) |
Store at 2~8°C protected from light, valid for 6 years. |
|
PMA |
Store at -25~-15°C protected from light, valid for 3 years. |
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
Documents
Manuals:
Related Products
|
Cat.NO. |
Product Name |
|
92639ES |
Human Organoids Cytokine Set |
|
92641ES |
Mouse BMDC Differentiation Cytokine Set |
| Mouse Macrophage BMDM Cytokine Set | |
| Human Dendritic Cell Differentiation Cytokine Set |
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