In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
Yeasen
Cleascrip ™ T7 RNA polimeraz (GMP sınıfı, düşük dsRNA, 250 U/μl) _ 10629es
Satış fiyatıİtibaren $198.00
Kaydetmek $40.00
Yeasen
mRNA kapağı 2'-o-metiltransferaz GMP derecesi (50 U/μl) _ 10612es
Satış fiyatıİtibaren $165.00
Kaydetmek $55.00
