Tanım
The HieffTM Pro Endo-free Plasmid Maxi Kit enables rapid purification of 0.5–4 mg of high-copy plasmid DNA from 50–200 mL bacterial culture (100–200 mL LB medium; 50–100 mL 2YT medium), with recovery rates reaching 80–90%. The kit is characterized by high efficiency, speed, and ease of use. The spin column utilizes a proprietary silica-based matrix material developed in-house, combined with a unique lysis buffer formulation, to maximize recovery of high-purity DNA. The inclusion of a push filter simplifies operation. Purified DNA exhibits high purity and extremely low endotoxin levels (<0.01 EU/μg DNA), making it suitable for standard and sensitive cell transfections, as well as direct applications in restriction digestion, transformation, PCR, in vitro transcription, sequencing, and other molecular biology experiments.
Features
High Binding Capacity – Silica membrane columns can bind up to 4 mg of plasmid DNA.
High Purity & Low Endotoxin – Plasmid endotoxin levels <0.01 EU/μg (for mid-to-high copy plasmids), significantly lower than conventional methods (>2.5 EU/μg).
Flexible Compatibility – Compatible with both high-speed angled rotors and high-throughput horizontal rotors.
Easy Operation – Push-filter design eliminates the need for careful supernatant handling.
Components
|
Part |
Components No. |
Name |
19038ES02 |
19038ES10 |
|
Part I |
19038-A |
RNase A (10 mg/mL) |
250 µL |
1200 µL |
|
Part II |
19038-B |
HieffTM DNA Column E2 |
2 |
10 |
|
19038-C |
50-mL Collection Tube |
2 |
10 |
|
|
19038-D |
Push Filter |
2 |
10 |
|
|
19038-E |
Buffer BS (Balance Solution)* |
10 mL |
50 mL |
|
|
19038-F |
Solution SI |
25 mL |
120 mL |
|
|
19038-G |
Solution SII |
25 mL |
120 mL |
|
|
19038-H |
Solution SIII |
20 mL |
100 mL |
|
|
19038-I |
Solution ER (Endotoxin Removal Solution) |
8 mL |
40 mL |
|
|
19038-J |
Solution BD (Binding Solution)* |
1.6 mL |
8 mL |
|
|
19038-K |
Wash Solution (Concentrated)* |
12 mL |
48 mL |
|
|
19038-L |
Elution Buffer (EB) |
10 mL |
30 mL |
|
|
19038-M |
Blue Buffer |
200 µL |
600 µL |
Storage
Part I: 19038-A (RNase A, 10 mg/mL), store at -15°C to -25°C. Stable for 18 months.
Part II: All other components, store at room temperature. Stable for 18 months
Application
Plasmid Extraction; Endo-free Plasmid Extraction.
Figures
1. Workflow
Figure 1. Workflow of the single-sample extraction kit, with a total processing time of ~54 minutes, from sample to purified product.
2. Higher plasmid yield
Result: The HieffTM Pro Endo-free Plasmid Maxi Kit (19038ES) achieved 1.26× higher plasmid yield compared with Supplier A*, while maintaining comparable purity.
3. Lower Endotoxin Levels
Figure 2. Plasmid extraction performance comparison.
The 19038ES kit consistently delivered plasmid DNA with endotoxin levels below 0.01 EU/µg, significantly lower than Supplier A*.
Documents:
Safety Data Sheet
Manuals
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Sorgu
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SSS
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