Tanım
The Hieff TransTM UltraProFect-CHO Transfection Reagent Kit is a highly efficient kit designed specifically for protein expression in CHO cells. It combines the powerful capability of rapid, ultra-high yield transient protein production in suspension culture with the exceptional advantages of the Chinese Hamster Ovary (CHO) cell expression system. Based on high-density CHO cell culture, this kit is paired with a transfection reagent meticulously designed for high-density CHO suspension culture, along with specialized enhancers and medium additives, achieving a synergistic effect on protein expression.
In various CHO cell lines (including expiCHO, CHO-S, CHO-K1, etc.), this kit achieves highly efficient protein expression. It performs exceptionally well in the production of complex proteins such as full-length monoclonal antibodies, nanobodies, and bispecific antibodies. It significantly improves expression efficiency and yield, meeting protein production requirements across diverse application scenarios.
Features
High Transfection Efficiency
Advanced targeted delivery ensures high DNA uptake and protein yield in CHO cells.
Broad Versatility
Works with ExpiCHO, CHO-S, and CHO-K1 cells for full-length, nanobody, and bispecific antibodies. Scales linearly from shake flasks to bioreactors.
Cost-Effective
Delivers performance comparable to leading brands at low price.
Specifications
|
Cat.No. |
40828ES80 |
|
Size |
1000 mL |
Components
|
Components No. |
Name |
40828ES80 |
Component Cat. No. |
|
40828-A |
UltraProFect Transfection Reagent |
4×1 mL |
40830ES |
|
40828-B |
TransBuffer Dilution Buffer |
100 mL |
40831ES |
|
40828-C |
CHO Serum-Free Medium |
1000 mL |
40161ES |
|
40828-D |
Enhancer |
20 mL |
40162ES |
|
40828-E |
Feed YS01 Supplement |
300 mL |
40163ES |
Storage
Store at 2~8°C, protected from light. Valid for 1 year.
Figure
High-Efficiency Protein Expression
(A) . Monoclonal Antibody Expression Comparison
(B) . Nanobody Expression Comparison
(C). Bispecific Antibody(BsAb) Expression Comparison
Figure 1. Performance comparison of Yeasen’s CHO Protein Expression Kit vs. leading brands in antibody yield, cell viability, and cell density.
Cell line: ExpiCHO, initial transfection density: 6×10⁶ cells/mL. Yeasen’s Hieff TransTM UltraPRO-CHO Transfection Reagent Kit achieves comparable antibody titers, cell viability, and cell growth profiles to leading brand reagents across full-length monoclonal antibodies, nanobodies, and bispecific antibodies, demonstrating robust and reliable performance for high-yield protein production.
Documents:
Safety Data Sheet
Manuals
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Sorgu
SSS
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