The Hieff™ Magnetic Circulating Cell-Free DNA Kit is suitable for extracting circulating DNA from 0.3-4 ml human plasma and serum. This product adopts unique magnetic beads and carefully optimized buffer system, which can maximize the purification and recovery of circulating DNA (typically 1-100 ng/ml circulating DNA in human plasma). The extracted nucleic acid is suitable for various downstream applications, such as NGS, qPCR, etc.

Feature

High Yield: Delivers high nucleic acid concentration with excellent recovery of short fragments.

Superior Quality: Produces high-quality libraries for downstream sequencing.

Broad Compatibility: Works with 0.3–4 mL plasma samples from both cfDNA-specific and standard EDTA tubes.

Components

Storage

Both components can be transported at room temperature. For storage, Component I should be kept at 2–8°C, while Component II can be stored at room temperature. Both have a shelf life of 18 months.

Figure

Case Study 1: Superior cfDNA Recovery from Healthy Donor Plasma

Using a 50 µL elution volume, the 18382 kit demonstrated outstanding recovery efficiency from 2 mL of healthy donor plasma collected in cfDNA preservation tubes. Quantitative analysis confirmed that the kit delivers high nucleic acid yields, ensuring sufficient template material for sensitive downstream molecular applications. 

Case Study 2: High-Integrity cfDNA Extraction from EDTA Plasma

Using a 20 µL elution volume, the 18382 kit achieved excellent recovery efficiency from 2 mL of healthy donor EDTA plasma. Fragment analysis revealed distinct characteristic peaks at 170 bp and 340 bp, confirming the high integrity of the extracted cfDNA. Notably, no high-molecular-weight DNA interference was observed, ensuring high-purity nucleic acids ready for downstream sequencing or PCR assays.

Figure 1. cfDNA yield and fragment analysis from 2 mL EDTA plasma using the 18382 kit.

Documents:

Safety Data Sheet

18386_MSDS_HB260730

Manuals

18386_Manual_HB20260730