The SuperMethyl™-Fast Bisulfite Conversion Kit from Ellis Bio offers the revolutionary fastest and most efficient bisulfite conversion solution available for DNA methylation analysis. Featuring a newly engineered, ready-to-use fast bisulfite conversion reagent and a magnetic bead-based DNA purification method, this kit streamlines the workflow by combining conversion and purification in one seamless process. Simply add the SuperMethyl™-Fast Bisulfite Conversion Reagent to your sample and incubate for 6 - 8 minutes at 98°C (standard protocol is 7 minutes).

Complete bisulfite conversion and DNA purification in under 1 hour, this kit not only delivers speed but also precision, achieving over 99.5% conversion efficiency while minimizing DNA degradation. Its versatility makes it indispensable for both research and clinical applications.

With an unmatched rapid workflow and exceptional performance, the SuperMethyl™-Fast Bisulfite Conversion Kit ensures reliable and reproducible results, making it the go-to choice for researchers who require both precision and quick turnaround times.

Features

• Fastest Conversion Available:10X faster than leading industry kits
• Exceptional Performance:Excellent methylation protection; Compatible with a wide range of DNA inputs.
• Versatile Applications:Optimized for PCR, microarrays, endonuclease digestion, and next-generation sequencing (NGS).
• Cost-Effective:At just $179.99, competitively priced with a price-match guarantee.

Components

* SuperMethyl - Fast Wash Buffer requires the addition of 5C mL 100% ethanol (EtOH) before first use.

Shipping Conditions

No Cold Chain Required; Shipped at Room Temperature

Precautions / Handling Instructions:

Upon receipt of the kit, store the Conversion Reagent at 4°C.

Workflow

Figures

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Figure 2. mESC gDNA converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. C-to-T conversion ratios were determined post-sequencing using unmethylated λ-DNA spike-in control.

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Figure 3. mESC gDNA with 1% unmethylated lambda DNA spike-in were converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. False positive rates were calculated post-NGS as the percentage of CpG and non-CpG cytosines showing >1% unconverted C.

4.Versatile and robust methyl-seq performance

Figure 4. Key performance metrics from whole-genome and targeted methylation profiling with the KAPA EvoPrep Kit and different conversion chemistries.

Documents:

Safety Data Sheet

MSDS

Manuals

Manual

Additional Resources:

SuperMethyl™ Fast Data Sheet

Microarray Data Brief