Hieff NGS™ MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) 2.0 utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.

Features

• Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
• Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
• High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
• Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.

Components

Applications

• Gene Expression Research
• Alternative splicing analysis
• Detection and discovery of LncRNA(Non coding RNA)
• dentification of selective polyadenylation sites
• Fusion gene detection

Storage

This product should be stored at -25~-15℃ for 12 months.

Figures

1. Broad Compatibility Across Input Amounts and Sample Types

Figure 1. Superior rRNA Removal Efficiency Across a Broad Input Range

Library preparation was performed using the MaxUp™ rRNA Depletion Kit and a strand-specific RNA Library Preparation Kit on human, mouse, and rat total RNA samples. Unlike traditional kits limited to 1 µg input, MaxUp™ demonstrates consistent and efficient rRNA removal across a wide range of input amounts (100 ng to 1 µg), achieving <1% rRNA residual. 

2. Effective rRNA Removal

 Figure 2. Comparison of rRNA Removal Efficiency: Depletion vs. Enrichment

Using 1 μg of 293T total RNA, samples were processed using either oligo-dT enrichment or rRNA depletion methods. Sequencing analysis revealed that both methods achieved comparable levels of rRNA removal efficiency.

3. Optimized for FFPOptimized for FFPE & Degraded Samples  

Figure 3. Robust rRNA Depletion Performance in FFPE Samples Across a Wide DV200 Range

MaxUp™ is optimized for challenging FFPE RNA, maintaining strong performance even with degraded transcripts. The kit demonstrates consistent and efficient rRNA removal across a broad DV200 range (20%–70%), achieving >98% depletion efficiency with a 100 ng input. This ensures high-quality, low-background library construction, making it highly compatible with low-quality samples (DV200 < 50%) for clinical and archival tissue research.

4. Automation-Ready Stability  

Figure 4. Enhanced Stability and Automation Compatibility 

MaxUp™ features a stabilized RNase H system that eliminates the need for fresh enzyme preparation, making it ideal for automated, high-throughput workflows. The kit maintains superior depletion efficiency (>95%) under various storage conditions, including extended periods at 37°C, RT, and 4°C.

Documents:

Safety Data Sheet

12726_MSDS_HB250805_EN.PDF

Manuals

12726_Manual_HB20260401.pdf

Related Blogs：

rRNA Depletion Kit – Efficient rRNA Removal Across All Sample Types