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The Hieff NGS™ Hyb & Wash Kit with Universal Blocking Oligo for Illumina is a capture hybridization and wash reagent specifically developed for YEASEN targeted capture product lines. It utilizes novel double-stranded DNA probes (100-mer) to capture and enrich target DNA for high-throughput sequencing. The carefully optimized single-tube hybridization buffer system delivers more stable and superior capture performance. With outstanding product performance, enhanced sensitivity, and specificity, the NGS targeted capture kit is widely applied in research areas such as novel molecular diagnostics and detection. All reagents provided in the kit have undergone rigorous quality control and functional validation, ensuring maximum experimental stability and reproducibility.
Specifications
|
Cat.No. |
12245ES04 / 12245ES24 / 12245ES96 |
|
Size |
4 T / 24 T / 96 T |
Components
|
Components No. |
Name |
12245ES04 |
12245ES24 |
12245ES96 |
|
|
BOX-I |
12245-A |
Hyb Human Block |
20 μL |
120 μL |
540 μL |
|
12245-B |
RNase Block |
20 μL |
120 μL |
540 μL |
|
|
12245-C |
Hyb Buffer v2 |
80 μL |
480 μL |
2*1080 μL |
|
|
BOX-II |
12245-D |
Binding Buffer |
3.5 mL |
21 mL |
84 mL |
|
12245-E |
Wash Buffer 2 v2 |
4 mL |
24 mL |
108 mL |
|
|
12245-F |
Wash Buffer 1 |
880 μL |
5.3 mL |
24 mL |
|
|
BOX-III |
12245-G |
Post PCR Master Mix |
110 μL |
660 μL |
2*1350 μL |
|
12245-H |
Post PCR Primer for Illumina (25 μM) |
8 μL |
48 μL |
192μL |
|
|
12245-I |
Universal Blocking Oligo for Illumina |
8 μL |
48 μL |
200 μL |
|
Note: RNase Block* prevents RNA degradation and is used for RNA probe capture panels. If using 12244ES (DNA probes), this component is not required.
Feature
Excellent Compatibility: Compatible with both DNA capture probes and RNA capture probes.
Broad Applicability: Suitable for multiple application areas including tumor companion diagnostics, drug efficacy assessment, genetic screening, and molecular breeding.
Easy Operation: Single-tube workflow with simple steps and good compatibility.
Low Equipment Dependence: Magnetic bead concentration can replace vacuum centrifugation.
Application Case
I. The Hieff NGS™ Hyb & Wash Kit is compatible with probes from other manufacturers, delivering more uniform data.
Uniformity performance of the Hieff NGS™ Hyb & Wash Kit hybridization and wash systemcombined with a competitor’s 3 Tumor Panel. Following the manufacturer’s operating instructions, sequencing was performed on the NovaSeq 6000 platform with PE150. The bar chart represents the size of regions with different GC content in the target area, and the curve represents the coverage depth of regions with different GC content.
Storage
BOX-I: Store at -25~-15°C. Valid for 1 year.
BOX-II: Store at room temperature. Valid for 1 year.
BOX-III: Store at -25~-15°C. Valid for 1 year.
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