Beskrivning
Booster DNA/RNA Transfection Reagent is an advanced nucleic acid transfection reagent developed based on novel polymeric materials. Its unique molecular design significantly reduces cytotoxicity and features a smart anti-enzymatic degradation mechanism that protects nucleic acids from intracellular enzymatic degradation during delivery, thereby maximizing transfection efficiency. This reagent is suitable for various types of nucleic acids including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for efficient transfection in cell lines such as 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW267.4, HCT116, as well as primary cells, providing reliable tools for gene research.
Features
Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.
Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).
Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells.
Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.
One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.
Components
|
Components No. |
Name |
40801ES01 |
40801ES04 |
|
40801-A |
Booster DNA/RNA Transfection Reagent |
100 μL |
1.5 mL |
|
40801-B |
Transfection Enhancer |
100 μL |
1.5 mL |
Shipping and Storage
This product should be stored at 2~8℃ for 12 months.
Application
Cell Transfection; DNA Transfection; mRNA/SiRNA Transfection;
Figures
1) High-Efficiency Primary Cell BMDM Transfection
Figure 1. Plasmid DNA transfection in primary bone marrow-derived macrophages (BMDMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
2) High-Efficiency DNA Transfection
Figure 2. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster.
3) High-Efficiency mRNA Transfection
Figure 3. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster.
4) High-Efficiency siRNA Transfection
Figure 4. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster.
Customer Feedback
1) High-Efficiency Primary Dermal Fibroblasts Transfection
Figure 5. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
2) High-Efficiency Porcine Alveolar Macrophages Transfection
Figure 6. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.
Documents:
Safety Data Sheet
Manuals
40801_Manual_Ver.EN20250807.pdf
Related Blog:
Protocol for Transfection of mRNA into Cells
Protocol for Transfection of siRNA into Cells
Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection Reagent
Protocol for Transfection of Primary Bone Marrow-Derived Macrophages (BMDMs)
Try it for free!
Get a free sample of Hieff Trans™ Booster and validate its performance in your own lab.
👉 [Request Sample Now]
Betalning och säkerhet
Din betalningsinformation behandlas säkert. Vi lagrar inte kreditkortsuppgifter och har inte heller tillgång till din kreditkortsinformation.
Förfrågan
Du kanske också gillar
Vanliga frågor
Produkten är endast avsedd för forskningsändamål och är inte avsedd för terapeutisk eller diagnostisk användning hos människor eller djur. Produkter och innehåll skyddas av patent, varumärken och upphovsrätt som ägs av Yeasen Biotechnology. Varumärkessymboler anger ursprungsland, inte nödvändigtvis registrering i alla regioner.
Vissa applikationer kan kräva ytterligare immateriella rättigheter från tredje part.
Yeasen är dedikerad till etisk vetenskap, och anser att vår forskning bör behandla kritiska frågor samtidigt som den säkerställer säkerhet och etiska standarder.