Descrição
Hieff NGSTM MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.
Features
- Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
- Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
- High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
- Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.
Components
|
Components No. |
Name |
12257ES12 |
12257ES24 |
12257ES96 |
|
12257-A |
Hybridization Buffer |
36 μL |
72 μL |
288 μL |
|
12257-B |
Human Probe Mix (rRNA & ITS/ETS) |
24 μL |
48 μL |
192 μL |
|
12257-C |
RNase H Buffer |
36 μL |
72 μL |
288 μL |
|
12257-D |
RNase H |
4 μL |
48 μL |
192 μL |
|
12257-E |
DNase I Buffer |
330 μL |
660 μL |
2x1320 μL |
|
12257-F |
DNase l |
30 μL |
60 μL |
240 μL |
Applications
- Gene Expression Research
- Alternative splicing analysis
- Detection and discovery of LncRNA(Non coding RNA)
- dentification of selective polyadenylation sites
- Fusion gene detection
Storage
This product should be stored at -25~-15℃ for 12 months.
Pagamento e segurança
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Investigação
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