Alternative Names

TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A

Species

Human

Expression Sequence

Leu72-Leu251

Accession

O95150-1

Tag

N-monomeric hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 44.87 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Mouse DR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFSF15, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA . 【ELISA Data】 Immobilized Human TNFSF15, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.