Descrição
HGF R is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development.
Specifications
|
Synonyms |
MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
|
Uniprot No. |
P08581-1 |
|
Source |
Recombinant Human HGF R/c-MET Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu25-Thr932. |
|
Molecular Weight |
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE . |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Product Data
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Investigação
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